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Characterization of prostatic epithelial cell lines derived from transgenic adenocarcinoma of the mouse prostate (TRAMP) model.

Publication ,  Journal Article
Foster, BA; Gingrich, JR; Kwon, ED; Madias, C; Greenberg, NM
Published in: Cancer Res
August 15, 1997

To develop a syngeneic transplantable system to study immunotherapeutic approaches for the treatment of prostate cancer, three cell lines were established from a heterogeneous 32 week tumor of the transgenic adenocarcinoma mouse prostate (TRAMP) model. TRAMP is a transgenic line of C57BL/6 mice harboring a construct comprised of the minimal -426/+28 rat probasin promoter driving prostate-specific epithelial expression of the SV40 large T antigen. TRAMP males develop histological prostatic intraepithelial neoplasia by 8-12 weeks of age that progress to adenocarcinoma with distant metastases by 24-30 weeks of age. The three cell lines (TRAMP-C1, TRAMP-C2, and TRAMP-C3) express cytokeratin, E-cadherin, and androgen receptor by immunohistochemical analysis and do not appear to have a mutated p53. Although TRAMP-C1 and TRAMP-C2 are tumorigenic when grafted into syngeneic C57BL/6 hosts, TRAMP-C3 grows readily in vitro but does not form tumors. The T antigen oncoprotein is not expressed by the cell lines in vitro or in vivo. The rationale for establishing multiple cell lines was to isolate cells representing various stages of cellular transformation and progression to androgen-independent metastatic disease that could be manipulated in vitro and, in combination with the TRAMP model, provide a system to investigate therapeutic interventions, such as immunotherapy prior to clinical trials.

Duke Scholars

Published In

Cancer Res

ISSN

0008-5472

Publication Date

August 15, 1997

Volume

57

Issue

16

Start / End Page

3325 / 3330

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Tumor Stem Cell Assay
  • Tumor Cells, Cultured
  • Receptors, Androgen
  • RNA, Messenger
  • Prostatic Neoplasms
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Mice, Transgenic
  • Mice, Inbred C57BL
 

Citation

APA
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ICMJE
MLA
NLM
Foster, B. A., Gingrich, J. R., Kwon, E. D., Madias, C., & Greenberg, N. M. (1997). Characterization of prostatic epithelial cell lines derived from transgenic adenocarcinoma of the mouse prostate (TRAMP) model. Cancer Res, 57(16), 3325–3330.
Foster, B. A., J. R. Gingrich, E. D. Kwon, C. Madias, and N. M. Greenberg. “Characterization of prostatic epithelial cell lines derived from transgenic adenocarcinoma of the mouse prostate (TRAMP) model.Cancer Res 57, no. 16 (August 15, 1997): 3325–30.
Foster BA, Gingrich JR, Kwon ED, Madias C, Greenberg NM. Characterization of prostatic epithelial cell lines derived from transgenic adenocarcinoma of the mouse prostate (TRAMP) model. Cancer Res. 1997 Aug 15;57(16):3325–30.
Foster BA, Gingrich JR, Kwon ED, Madias C, Greenberg NM. Characterization of prostatic epithelial cell lines derived from transgenic adenocarcinoma of the mouse prostate (TRAMP) model. Cancer Res. 1997 Aug 15;57(16):3325–3330.

Published In

Cancer Res

ISSN

0008-5472

Publication Date

August 15, 1997

Volume

57

Issue

16

Start / End Page

3325 / 3330

Location

United States

Related Subject Headings

  • Tumor Suppressor Protein p53
  • Tumor Stem Cell Assay
  • Tumor Cells, Cultured
  • Receptors, Androgen
  • RNA, Messenger
  • Prostatic Neoplasms
  • Oncology & Carcinogenesis
  • Neoplasm Proteins
  • Mice, Transgenic
  • Mice, Inbred C57BL