Whole-brain activity mapping onto a zebrafish brain atlas.

Journal Article (Journal Article)

In order to localize the neural circuits involved in generating behaviors, it is necessary to assign activity onto anatomical maps of the nervous system. Using brain registration across hundreds of larval zebrafish, we have built an expandable open-source atlas containing molecular labels and definitions of anatomical regions, the Z-Brain. Using this platform and immunohistochemical detection of phosphorylated extracellular signal–regulated kinase (ERK) as a readout of neural activity, we have developed a system to create and contextualize whole-brain maps of stimulus- and behavior-dependent neural activity. This mitogen-activated protein kinase (MAP)-mapping assay is technically simple, and data analysis is completely automated. Because MAP-mapping is performed on freely swimming fish, it is applicable to studies of nearly any stimulus or behavior. Here we demonstrate our high-throughput approach using pharmacological, visual and noxious stimuli, as well as hunting and feeding. The resultant maps outline hundreds of areas associated with behaviors.

Full Text

Duke Authors

Cited Authors

  • Randlett, O; Wee, CL; Naumann, EA; Nnaemeka, O; Schoppik, D; Fitzgerald, JE; Portugues, R; Lacoste, AMB; Riegler, C; Engert, F; Schier, AF

Published Date

  • November 2015

Published In

Volume / Issue

  • 12 / 11

Start / End Page

  • 1039 - 1046

PubMed ID

  • 26778924

Pubmed Central ID

  • PMC4710481

Electronic International Standard Serial Number (EISSN)

  • 1548-7105

Digital Object Identifier (DOI)

  • 10.1038/nmeth.3581


  • eng

Conference Location

  • United States