S-nitrosylation drives cell senescence and aging in mammals by controlling mitochondrial dynamics and mitophagy.


Journal Article

S-nitrosylation, a prototypic redox-based posttranslational modification, is frequently dysregulated in disease. S-nitrosoglutathione reductase (GSNOR) regulates protein S-nitrosylation by functioning as a protein denitrosylase. Deficiency of GSNOR results in tumorigenesis and disrupts cellular homeostasis broadly, including metabolic, cardiovascular, and immune function. Here, we demonstrate that GSNOR expression decreases in primary cells undergoing senescence, as well as in mice and humans during their life span. In stark contrast, exceptionally long-lived individuals maintain GSNOR levels. We also show that GSNOR deficiency promotes mitochondrial nitrosative stress, including excessive S-nitrosylation of Drp1 and Parkin, thereby impairing mitochondrial dynamics and mitophagy. Our findings implicate GSNOR in mammalian longevity, suggest a molecular link between protein S-nitrosylation and mitochondria quality control in aging, and provide a redox-based perspective on aging with direct therapeutic implications.

Full Text

Duke Authors

Cited Authors

  • Rizza, S; Cardaci, S; Montagna, C; Di Giacomo, G; De Zio, D; Bordi, M; Maiani, E; Campello, S; Borreca, A; Puca, AA; Stamler, JS; Cecconi, F; Filomeni, G

Published Date

  • April 2018

Published In

Volume / Issue

  • 115 / 15

Start / End Page

  • E3388 - E3397

PubMed ID

  • 29581312

Pubmed Central ID

  • 29581312

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.1722452115


  • eng