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Emmission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension

Publication ,  Journal Article
Gardner, SY; McGee, JK; Kodavanti, UP; Ledbetter, A; Everitt, JI; Winsett, DW; Doerfler, DL; Costa, DL
Published in: Environmental Health Perspectives
January 1, 2004

Preexistent cardiopulmonary disease in humans appears to enhance susceptibility to the adverse effects of ambient particulate matter. Previous studies in this laboratory have demonstrated enhanced inflammation and mortality after intratracheal instillation (IT) and inhalation (INH) of residual oil fly ash (ROFA) in a rat model of pulmonary hypertension induced by monocrotaline (MCT). The present study was conducted to examine the effects of ROFA in this model on ventilatory function in unanesthetized, unrestrained animals. Sixty-day-old male CD rats were injected with MCT (60 mg/kg) or vehicle (VEH) intraperitoneally 10 days before IT of ROFA (8.3 mg/kg) or saline (SAL) (control) or nose-only INH of ROFA [15 Mg/m3 for 6 hr on 3 consecutive days or air (control)]. At 24 and 72 hr after exposure, rats were studied individually in a simultaneous gas uptake/whole-body plethysmograph. Lungs were removed at 72 hr for histology. Pulmonary test results showed that tidal volume (VT) decreased 24 hr after IT of ROFA in MCT-treated rats. Breathing frequency, minute volume (VE), and the ventilatory equivalent for oxygen increased in MCT- and VEH-treated rats 24 hr after IT or INH of ROFA and remained elevated 72 hr post-IT. O2 uptake (VO2) decreased after IT of ROFA in MCT-treated rats. Carbon monoxide uptake decreased 24 hr after IT of ROFA, returning to control values in VEH-treated rats but remaining low in MCT-treated rats 72 hr post-IT. ROFA exposure induced histologic changes and abnormalities in several ventilatory parameters, many of which were enhanced by MCT treatment.

Duke Scholars

Published In

Environmental Health Perspectives

DOI

ISSN

0091-6765

Publication Date

January 1, 2004

Volume

112

Issue

8

Start / End Page

872 / 878

Related Subject Headings

  • Toxicology
  • 11 Medical and Health Sciences
  • 05 Environmental Sciences
 

Citation

APA
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ICMJE
MLA
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Gardner, S. Y., McGee, J. K., Kodavanti, U. P., Ledbetter, A., Everitt, J. I., Winsett, D. W., … Costa, D. L. (2004). Emmission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension. Environmental Health Perspectives, 112(8), 872–878. https://doi.org/10.1289/ehp.6583
Gardner, S. Y., J. K. McGee, U. P. Kodavanti, A. Ledbetter, J. I. Everitt, D. W. Winsett, D. L. Doerfler, and D. L. Costa. “Emmission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension.” Environmental Health Perspectives 112, no. 8 (January 1, 2004): 872–78. https://doi.org/10.1289/ehp.6583.
Gardner SY, McGee JK, Kodavanti UP, Ledbetter A, Everitt JI, Winsett DW, et al. Emmission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension. Environmental Health Perspectives. 2004 Jan 1;112(8):872–8.
Gardner, S. Y., et al. “Emmission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension.” Environmental Health Perspectives, vol. 112, no. 8, Jan. 2004, pp. 872–78. Scopus, doi:10.1289/ehp.6583.
Gardner SY, McGee JK, Kodavanti UP, Ledbetter A, Everitt JI, Winsett DW, Doerfler DL, Costa DL. Emmission-particle-induced ventilatory abnormalities in a rat model of pulmonary hypertension. Environmental Health Perspectives. 2004 Jan 1;112(8):872–878.

Published In

Environmental Health Perspectives

DOI

ISSN

0091-6765

Publication Date

January 1, 2004

Volume

112

Issue

8

Start / End Page

872 / 878

Related Subject Headings

  • Toxicology
  • 11 Medical and Health Sciences
  • 05 Environmental Sciences