Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies.
Journal Article (Journal Article)
The design, formulation, and immunological evaluation of self-assembling peptide materials is relatively straightforward. Indeed, one of the advantages of synthetic self-assembling peptides is that one can progress from initial concept to in vivo testing in a matter of days. However, because these materials are supramolecular, working with them is not without some practical challenges, and subtle changes in design, synthesis, handling, and formulation can affect the materials' immunogenicity. This chapter is intended to communicate some of these practical aspects of working with these materials that are not always enumerated in conventional research papers. Epitope considerations, peptide synthesis, purification, storage, nanofiber formation, quality control, immunological evaluation, and the overall phenotypic characteristics of the immune responses to be expected from these materials are discussed.
Full Text
Duke Authors
Cited Authors
- Solano, CM; Wen, Y; Han, H; Collier, JH
Published Date
- January 2018
Published In
Volume / Issue
- 1777 /
Start / End Page
- 233 - 248
PubMed ID
- 29744839
Electronic International Standard Serial Number (EISSN)
- 1940-6029
International Standard Serial Number (ISSN)
- 1064-3745
Digital Object Identifier (DOI)
- 10.1007/978-1-4939-7811-3_14
Language
- eng