Practical Considerations in the Design and Use of Immunologically Active Fibrillar Peptide Assemblies.

Published

Journal Article

The design, formulation, and immunological evaluation of self-assembling peptide materials is relatively straightforward. Indeed, one of the advantages of synthetic self-assembling peptides is that one can progress from initial concept to in vivo testing in a matter of days. However, because these materials are supramolecular, working with them is not without some practical challenges, and subtle changes in design, synthesis, handling, and formulation can affect the materials' immunogenicity. This chapter is intended to communicate some of these practical aspects of working with these materials that are not always enumerated in conventional research papers. Epitope considerations, peptide synthesis, purification, storage, nanofiber formation, quality control, immunological evaluation, and the overall phenotypic characteristics of the immune responses to be expected from these materials are discussed.

Full Text

Duke Authors

Cited Authors

  • Solano, CM; Wen, Y; Han, H; Collier, JH

Published Date

  • January 2018

Published In

Volume / Issue

  • 1777 /

Start / End Page

  • 233 - 248

PubMed ID

  • 29744839

Pubmed Central ID

  • 29744839

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-7811-3_14

Language

  • eng