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Enhanced Glucose Control Following Vertical Sleeve Gastrectomy Does Not Require a β-Cell Glucagon-Like Peptide 1 Receptor.

Publication ,  Journal Article
Douros, JD; Lewis, AG; Smith, EP; Niu, J; Capozzi, M; Wittmann, A; Campbell, J; Tong, J; Wagner, C; Mahbod, P; Seeley, R; D'Alessio, DA
Published in: Diabetes
August 2018

Bariatric surgeries, including vertical sleeve gastrectomy (VSG), resolve diabetes in 40-50% of patients. Studies examining the molecular mechanisms underlying this effect have centered on the role of the insulinotropic glucagon-like peptide 1 (GLP-1), in great part because of the ∼10-fold rise in its circulating levels after surgery. However, there is currently debate over the role of direct β-cell signaling by GLP-1 to mediate improved glucose tolerance following surgery. In order to assess the importance of β-cell GLP-1 receptor (GLP-1R) for improving glucose control after VSG, a mouse model of this procedure was developed and combined with a genetically modified mouse line allowing an inducible, β-cell-specific Glp1r knockdown (Glp1rβ-cell-ko). Mice with VSG lost ∼20% of body weight over 30 days compared with sham-operated controls and had a ∼60% improvement in glucose tolerance. Isolated islets from VSG mice had significantly greater insulin responses to glucose than controls. Glp1r knockdown in β-cells caused glucose intolerance in diet-induced obese mice compared with obese controls, but VSG improved glycemic profiles to similar levels during oral and intraperitoneal glucose challenges in Glp1rβ-cell-ko and Glp1rWT mice. Therefore, even though the β-cell GLP-1R seems to be important for maintaining glucose tolerance in obese mice, in these experiments it is dispensable for the improvement in glucose tolerance after VSG. Moreover, the metabolic physiology activated by VSG can overcome the deficits in glucose regulation caused by lack of β-cell GLP-1 signaling in obesity.

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Published In

Diabetes

DOI

EISSN

1939-327X

Publication Date

August 2018

Volume

67

Issue

8

Start / End Page

1504 / 1511

Location

United States

Related Subject Headings

  • Weight Loss
  • Tissue Culture Techniques
  • Signal Transduction
  • Organ Specificity
  • Obesity
  • Mice, Transgenic
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Male
  • Insulin-Secreting Cells
 

Citation

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Douros, J. D., Lewis, A. G., Smith, E. P., Niu, J., Capozzi, M., Wittmann, A., … D’Alessio, D. A. (2018). Enhanced Glucose Control Following Vertical Sleeve Gastrectomy Does Not Require a β-Cell Glucagon-Like Peptide 1 Receptor. Diabetes, 67(8), 1504–1511. https://doi.org/10.2337/db18-0081
Douros, Jonathan D., Alfor G. Lewis, Eric P. Smith, JingJing Niu, Megan Capozzi, April Wittmann, Jonathan Campbell, et al. “Enhanced Glucose Control Following Vertical Sleeve Gastrectomy Does Not Require a β-Cell Glucagon-Like Peptide 1 Receptor.Diabetes 67, no. 8 (August 2018): 1504–11. https://doi.org/10.2337/db18-0081.
Douros JD, Lewis AG, Smith EP, Niu J, Capozzi M, Wittmann A, et al. Enhanced Glucose Control Following Vertical Sleeve Gastrectomy Does Not Require a β-Cell Glucagon-Like Peptide 1 Receptor. Diabetes. 2018 Aug;67(8):1504–11.
Douros, Jonathan D., et al. “Enhanced Glucose Control Following Vertical Sleeve Gastrectomy Does Not Require a β-Cell Glucagon-Like Peptide 1 Receptor.Diabetes, vol. 67, no. 8, Aug. 2018, pp. 1504–11. Pubmed, doi:10.2337/db18-0081.
Douros JD, Lewis AG, Smith EP, Niu J, Capozzi M, Wittmann A, Campbell J, Tong J, Wagner C, Mahbod P, Seeley R, D’Alessio DA. Enhanced Glucose Control Following Vertical Sleeve Gastrectomy Does Not Require a β-Cell Glucagon-Like Peptide 1 Receptor. Diabetes. 2018 Aug;67(8):1504–1511.

Published In

Diabetes

DOI

EISSN

1939-327X

Publication Date

August 2018

Volume

67

Issue

8

Start / End Page

1504 / 1511

Location

United States

Related Subject Headings

  • Weight Loss
  • Tissue Culture Techniques
  • Signal Transduction
  • Organ Specificity
  • Obesity
  • Mice, Transgenic
  • Mice, Knockout
  • Mice, Inbred C57BL
  • Male
  • Insulin-Secreting Cells