Synthesis of Reconfigurable Flow-Based Biochips for Scalable Single-Cell Screening



© 1982-2012 IEEE. Single-cell screening is used to sort a stream of cells into clusters (or types) based on prespecified biomarkers, thus supporting type-driven biochemical analysis. Reconfigurable flow-based microfluidic biochips (RFBs) can be utilized to screen hundreds of heterogeneous cells within a few minutes, but they are overburdened with the control of a large number of valves. To address this problem, we present a pin-constrained RFB design methodology for single-cell screening. The proposed design is analyzed using computational fluid dynamics simulations, mapped to an RC-lumped model, and combined with intervalve connectivity information to construct a high-level synthesis framework, referred to as cell sorter using multiplexed control (Sortex). Simulation results show that Sortex significantly reduces the number of control pins and fulfills the timing requirements of single-cell screening.

Full Text

Cited Authors

  • Ibrahim, M; Sridhar, A; Chakrabarty, K; Schlichtmann, U

Published Date

  • December 1, 2019

Published In

Start / End Page

  • 2255 - 2270

Electronic International Standard Serial Number (EISSN)

  • 1937-4151

International Standard Serial Number (ISSN)

  • 0278-0070

Digital Object Identifier (DOI)

  • 10.1109/TCAD.2018.2878159

Citation Source

  • Scopus