Detection of phytoestrogens in samples of second trimester human amniotic fluid.

Journal Article (Journal Article)

There is widespread concern that fetal exposure to hormonally active chemicals may adversely affect development of the reproductive tract. Therefore, the present study was performed to develop the necessary analytical methods and test the hypothesis that dietary phytoestrogens can be quantified in second trimester human amniotic fluid. Amniotic fluid samples (n=59) from women (n=53) undergoing routine amniocentesis between 15 and 23 weeks of gestation were analyzed by gas chromatography/mass spectrometric (GC/MS). Analytes included the phytoestrogens daidzein, genistein, formononetin, biochanin A, and coumestrol. Dietary phytoestrogens were quantified in 96.2% of second trimester amniotic fluid samples tested. The mean (+/- standard deviation (S.D.)) concentration of daidzein and genistein in amniotic fluid was 1.44 +/- 1.34 and 1.69 +/- 1.48 ng/ml with maximum levels of 5.52 and 6.54 ng/ml, respectively. Second trimester amniotic fluid contains quantifiable levels of dietary phytoestrogens and thus is a marker of mid pregnancy fetal exposure.

Full Text

Duke Authors

Cited Authors

  • Foster, WG; Chan, S; Platt, L; Hughes, CL

Published Date

  • March 28, 2002

Published In

Volume / Issue

  • 129 / 3

Start / End Page

  • 199 - 205

PubMed ID

  • 11888703

International Standard Serial Number (ISSN)

  • 0378-4274

Digital Object Identifier (DOI)

  • 10.1016/s0378-4274(02)00018-8


  • eng

Conference Location

  • Netherlands