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Genetic Control of Genomic Alterations Induced in Yeast by Interstitial Telomeric Sequences.

Publication ,  Journal Article
Moore, A; Dominska, M; Greenwell, P; Aksenova, AY; Mirkin, S; Petes, T
Published in: Genetics
June 2018

In many organisms, telomeric sequences can be located internally on the chromosome in addition to their usual positions at the ends of the chromosome. In humans, such interstitial telomeric sequences (ITSs) are nonrandomly associated with translocation breakpoints in tumor cells and with chromosome fragile sites (regions of the chromosome that break in response to perturbed DNA replication). We previously showed that ITSs in yeast generated several different types of instability, including terminal inversions (recombination between the ITS and the "true" chromosome telomere) and point mutations in DNA sequences adjacent to the ITS. In the current study, we examine the genetic control of these events. We show that the terminal inversions occur by the single-strand annealing pathway of DNA repair following the formation of a double-stranded DNA break within the ITS. The point mutations induced by the ITS require the error-prone DNA polymerase ζ. Unlike the terminal inversions, these events are not initiated by a double-stranded DNA break, but likely result from the error-prone repair of a single-stranded DNA gap or recruitment of DNA polymerase ζ in the absence of DNA damage.

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Published In

Genetics

DOI

EISSN

1943-2631

Publication Date

June 2018

Volume

209

Issue

2

Start / End Page

425 / 438

Location

United States

Related Subject Headings

  • Telomere
  • Sequence Inversion
  • Saccharomyces cerevisiae
  • Recombinational DNA Repair
  • Genomic Instability
  • Genome, Fungal
  • Developmental Biology
  • DNA-Directed DNA Polymerase
  • DNA Breaks, Double-Stranded
  • 3105 Genetics
 

Citation

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Moore, A., Dominska, M., Greenwell, P., Aksenova, A. Y., Mirkin, S., & Petes, T. (2018). Genetic Control of Genomic Alterations Induced in Yeast by Interstitial Telomeric Sequences. Genetics, 209(2), 425–438. https://doi.org/10.1534/genetics.118.300950
Moore, Anthony, Margaret Dominska, Patricia Greenwell, Anna Y. Aksenova, Sergei Mirkin, and Thomas Petes. “Genetic Control of Genomic Alterations Induced in Yeast by Interstitial Telomeric Sequences.Genetics 209, no. 2 (June 2018): 425–38. https://doi.org/10.1534/genetics.118.300950.
Moore A, Dominska M, Greenwell P, Aksenova AY, Mirkin S, Petes T. Genetic Control of Genomic Alterations Induced in Yeast by Interstitial Telomeric Sequences. Genetics. 2018 Jun;209(2):425–38.
Moore, Anthony, et al. “Genetic Control of Genomic Alterations Induced in Yeast by Interstitial Telomeric Sequences.Genetics, vol. 209, no. 2, June 2018, pp. 425–38. Pubmed, doi:10.1534/genetics.118.300950.
Moore A, Dominska M, Greenwell P, Aksenova AY, Mirkin S, Petes T. Genetic Control of Genomic Alterations Induced in Yeast by Interstitial Telomeric Sequences. Genetics. 2018 Jun;209(2):425–438.

Published In

Genetics

DOI

EISSN

1943-2631

Publication Date

June 2018

Volume

209

Issue

2

Start / End Page

425 / 438

Location

United States

Related Subject Headings

  • Telomere
  • Sequence Inversion
  • Saccharomyces cerevisiae
  • Recombinational DNA Repair
  • Genomic Instability
  • Genome, Fungal
  • Developmental Biology
  • DNA-Directed DNA Polymerase
  • DNA Breaks, Double-Stranded
  • 3105 Genetics