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Environmental Stability of Plasmonic Biosensors Based on Natural versus Artificial Antibody.

Publication ,  Journal Article
Luan, J; Xu, T; Cashin, J; Morrissey, JJ; Kharasch, ED; Singamaneni, S
Published in: Anal Chem
July 3, 2018

Plasmonic biosensors based on the refractive index sensitivity of localized surface plasmon resonance (LSPR) are considered to be highly promising for on-chip and point-of-care biodiagnostics. However, most of the current plasmonic biosensors employ natural antibodies as biorecognition elements, which can easily lose their biorecognition ability upon exposure to environmental stressors (e.g., temperature and humidity). Plasmonic biosensors relying on molecular imprints as recognition elements (artificial antibodies) are hypothesized to be an attractive alternative for applications in resource-limited settings due to their excellent thermal, chemical, and environmental stability. In this work, we provide a comprehensive comparison of the stability of plasmonic biosensors based on natural and artificial antibodies. Although the natural antibody-based plasmonic biosensors exhibit superior sensitivity, their stability (temporal, thermal, and chemical) was found to be vastly inferior to those based on artificial antibodies. Our results convincingly demonstrate that these novel classes of artificial antibody-based plasmonic biosensors are highly attractive for point-of-care and resource-limited conditions where tight control over transport, storage, and handling conditions is not possible.

Duke Scholars

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Published In

Anal Chem

DOI

EISSN

1520-6882

Publication Date

July 3, 2018

Volume

90

Issue

13

Start / End Page

7880 / 7887

Location

United States

Related Subject Headings

  • Surface Plasmon Resonance
  • Protein Stability
  • Polymers
  • Molecular Imprinting
  • Gold
  • Biomimetic Materials
  • Antibodies
  • Analytical Chemistry
  • Adsorption
  • 4004 Chemical engineering
 

Citation

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Luan, J., Xu, T., Cashin, J., Morrissey, J. J., Kharasch, E. D., & Singamaneni, S. (2018). Environmental Stability of Plasmonic Biosensors Based on Natural versus Artificial Antibody. Anal Chem, 90(13), 7880–7887. https://doi.org/10.1021/acs.analchem.7b05470
Luan, Jingyi, Ting Xu, John Cashin, Jeremiah J. Morrissey, Evan D. Kharasch, and Srikanth Singamaneni. “Environmental Stability of Plasmonic Biosensors Based on Natural versus Artificial Antibody.Anal Chem 90, no. 13 (July 3, 2018): 7880–87. https://doi.org/10.1021/acs.analchem.7b05470.
Luan J, Xu T, Cashin J, Morrissey JJ, Kharasch ED, Singamaneni S. Environmental Stability of Plasmonic Biosensors Based on Natural versus Artificial Antibody. Anal Chem. 2018 Jul 3;90(13):7880–7.
Luan, Jingyi, et al. “Environmental Stability of Plasmonic Biosensors Based on Natural versus Artificial Antibody.Anal Chem, vol. 90, no. 13, July 2018, pp. 7880–87. Pubmed, doi:10.1021/acs.analchem.7b05470.
Luan J, Xu T, Cashin J, Morrissey JJ, Kharasch ED, Singamaneni S. Environmental Stability of Plasmonic Biosensors Based on Natural versus Artificial Antibody. Anal Chem. 2018 Jul 3;90(13):7880–7887.
Journal cover image

Published In

Anal Chem

DOI

EISSN

1520-6882

Publication Date

July 3, 2018

Volume

90

Issue

13

Start / End Page

7880 / 7887

Location

United States

Related Subject Headings

  • Surface Plasmon Resonance
  • Protein Stability
  • Polymers
  • Molecular Imprinting
  • Gold
  • Biomimetic Materials
  • Antibodies
  • Analytical Chemistry
  • Adsorption
  • 4004 Chemical engineering