Inhibitory proteins in the Newcastle disease virus-induced suppression of cell protein synthesis.

Published

Journal Article

Bolognesi, D. P. (Rensselaer Polytechnic Institute, Troy, N.Y.), and D. E. Wilson. Inhibitory proteins in the Newcastle disease virus-induced suppression of cell protein synthesis. J. Bacteriol. 91:1896-1901. 1966.-Infection by Newcastle disease virus brings about a rapid and marked inhibition of cell protein synthesis (CPS) in chick embryo fibroblast monolayers. The block to CPS is initiated about 5 hr after infection, and by 9 hr about 85% of the host protein synthesis is shut off. Azauridine (3 mg/ml), a ribonucleic acid (RNA) synthesis inhibitor, prevents the virus-induced inhibition of CPS when added at the time of infection; but it does not prevent the inhibition when added at 3 hr after infection. When puromycin (60 mug/ml), a protein synthesis inhibitor, was added at 3.5 hr after infection, viral RNA was synthesized in normal amounts, but the virus-induced inhibition of CPS was prevented. Actinomycin D added at the time of infection does not, however, prevent the virus-induced inhibition of CPS. The results of these experiments indicate that proteins synthesized during Newcastle disease virus replication are responsible for the inhibition of host-cell protein synthesis. The synthesis of these inhibitory proteins depends on the prior synthesis of viral RNA.

Full Text

Duke Authors

Cited Authors

  • Bolognesi, DP; Wilson, DE

Published Date

  • May 1, 1966

Published In

Volume / Issue

  • 91 / 5

Start / End Page

  • 1896 - 1901

PubMed ID

  • 5937245

Pubmed Central ID

  • 5937245

International Standard Serial Number (ISSN)

  • 0021-9193

Language

  • eng

Conference Location

  • United States