Determinants of antibody response after recombinant gp160 boosting in vaccinia-naive volunteers primed with gp160-recombinant vaccinia virus. The National Institute of Allergy and Infectious Diseases AIDS Vaccine Clinical Trials Network.
Journal Article (Clinical Trial;Journal Article;Multicenter Study)
Priming with a live recombinant vector followed by subunit boosting is a promising strategy for human immunodeficiency virus (HIV) immunization. Twenty-nine vaccinia-naive volunteers were primed with gp160-recombinant vaccinia virus (HIVAC-1e) and boosted with recombinant (r) gp160 to define factors associated with the magnitude and specificity of antibody response after booster immunization. A longer interval between inoculation and boost, two inoculations of HIVAC-1e with lesion formation occurring after the first, and Western blot-detectable antibody to gp160 after inoculation were significantly associated with higher neutralizing antibody titers and fusion-inhibiting activity after boosting. HIVAC-1e-primed vaccinees were more likely to have antibody to V3- and CD4-binding regions of gp120 and less likely to have antibody to constant regions 2 and 3 than vaccinees immunized with rgp160 alone. Priming volunteers with HIVAC-1e was a key determinant of the epitope specificity and magnitude of functional antibody responses induced by rgp160 boosting.
Full Text
Duke Authors
Cited Authors
- Graham, BS; Gorse, GJ; Schwartz, DH; Keefer, MC; McElrath, MJ; Matthews, TJ; Wright, PF; Belshe, RB; Clements, ML; Dolin, R
Published Date
- October 1994
Published In
Volume / Issue
- 170 / 4
Start / End Page
- 782 - 786
PubMed ID
- 7930718
International Standard Serial Number (ISSN)
- 0022-1899
Digital Object Identifier (DOI)
- 10.1093/infdis/170.4.782
Language
- eng
Conference Location
- United States