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Determinants of antibody response after recombinant gp160 boosting in vaccinia-naive volunteers primed with gp160-recombinant vaccinia virus. The National Institute of Allergy and Infectious Diseases AIDS Vaccine Clinical Trials Network.

Publication ,  Journal Article
Graham, BS; Gorse, GJ; Schwartz, DH; Keefer, MC; McElrath, MJ; Matthews, TJ; Wright, PF; Belshe, RB; Clements, ML; Dolin, R
Published in: J Infect Dis
October 1994

Priming with a live recombinant vector followed by subunit boosting is a promising strategy for human immunodeficiency virus (HIV) immunization. Twenty-nine vaccinia-naive volunteers were primed with gp160-recombinant vaccinia virus (HIVAC-1e) and boosted with recombinant (r) gp160 to define factors associated with the magnitude and specificity of antibody response after booster immunization. A longer interval between inoculation and boost, two inoculations of HIVAC-1e with lesion formation occurring after the first, and Western blot-detectable antibody to gp160 after inoculation were significantly associated with higher neutralizing antibody titers and fusion-inhibiting activity after boosting. HIVAC-1e-primed vaccinees were more likely to have antibody to V3- and CD4-binding regions of gp120 and less likely to have antibody to constant regions 2 and 3 than vaccinees immunized with rgp160 alone. Priming volunteers with HIVAC-1e was a key determinant of the epitope specificity and magnitude of functional antibody responses induced by rgp160 boosting.

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Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

October 1994

Volume

170

Issue

4

Start / End Page

782 / 786

Location

United States

Related Subject Headings

  • Viral Vaccines
  • Vaccinia virus
  • Vaccines, Synthetic
  • Recombinant Proteins
  • Protein Precursors
  • Middle Aged
  • Microbiology
  • Immunization, Secondary
  • Humans
  • HIV Seronegativity
 

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Graham, B. S., Gorse, G. J., Schwartz, D. H., Keefer, M. C., McElrath, M. J., Matthews, T. J., … Dolin, R. (1994). Determinants of antibody response after recombinant gp160 boosting in vaccinia-naive volunteers primed with gp160-recombinant vaccinia virus. The National Institute of Allergy and Infectious Diseases AIDS Vaccine Clinical Trials Network. J Infect Dis, 170(4), 782–786. https://doi.org/10.1093/infdis/170.4.782
Graham, B. S., G. J. Gorse, D. H. Schwartz, M. C. Keefer, M. J. McElrath, T. J. Matthews, P. F. Wright, R. B. Belshe, M. L. Clements, and R. Dolin. “Determinants of antibody response after recombinant gp160 boosting in vaccinia-naive volunteers primed with gp160-recombinant vaccinia virus. The National Institute of Allergy and Infectious Diseases AIDS Vaccine Clinical Trials Network.J Infect Dis 170, no. 4 (October 1994): 782–86. https://doi.org/10.1093/infdis/170.4.782.
Journal cover image

Published In

J Infect Dis

DOI

ISSN

0022-1899

Publication Date

October 1994

Volume

170

Issue

4

Start / End Page

782 / 786

Location

United States

Related Subject Headings

  • Viral Vaccines
  • Vaccinia virus
  • Vaccines, Synthetic
  • Recombinant Proteins
  • Protein Precursors
  • Middle Aged
  • Microbiology
  • Immunization, Secondary
  • Humans
  • HIV Seronegativity