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Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120.

Publication ,  Journal Article
Rusche, JR; Javaherian, K; McDanal, C; Petro, J; Lynn, DL; Grimaila, R; Langlois, A; Gallo, RC; Arthur, LO; Fischinger, PJ
Published in: Proc Natl Acad Sci U S A
May 1988
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Antisera to recombinant human immunodeficiency virus (HIV) proteins containing the entire envelope, gp160, or the central portion of the envelope, PB1, can inhibit fusion of virally infected cells in culture. This fusion inhibition is HIV-variant specific--that is, anti-gp160-IIIB inhibits fusion of isolate HTLV-IIIB-infected cells but not of isolate HTLV-IIIRF-infected cells. Both anti-gp160 and anti-PB1 are completely blocked in fusion inhibition activity by the addition of PB1 protein. A 24-amino acid peptide (RP135, amino acids 307-330) completely blocks fusion inhibition activity of both antisera and also blocks the activity of serum from a chimpanzee infected with HTLV-IIIB. Thus, the principal epitope that elicits fusion-inhibiting antibodies is located in the central portion of gp120.

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Dani Paul Bolognesi
Author Dani Paul Bolognesi Surgery, Surgical Sciences
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Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.85.9.3198

ISSN

0027-8424

Publication Date

May 1988

Volume

85

Issue

9

Start / End Page

3198 / 3202

Location

United States

Related Subject Headings

  • Viral Fusion Proteins
  • Retroviridae Proteins
  • Pan troglodytes
  • HIV Envelope Protein gp120
  • HIV
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay
  • Blood
  • Animals
  • Amino Acid Sequence
 

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Rusche, J. R., Javaherian, K., McDanal, C., Petro, J., Lynn, D. L., Grimaila, R., … Fischinger, P. J. (1988). Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120. Proc Natl Acad Sci U S A, 85(9), 3198–3202. https://doi.org/10.1073/pnas.85.9.3198
Rusche, J. R., K. Javaherian, C. McDanal, J. Petro, D. L. Lynn, R. Grimaila, A. Langlois, R. C. Gallo, L. O. Arthur, and P. J. Fischinger. “Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120.” Proc Natl Acad Sci U S A 85, no. 9 (May 1988): 3198–3202. https://doi.org/10.1073/pnas.85.9.3198.
Rusche JR, Javaherian K, McDanal C, Petro J, Lynn DL, Grimaila R, et al. Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120. Proc Natl Acad Sci U S A. 1988 May;85(9):3198–202.
Rusche, J. R., et al. “Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120.” Proc Natl Acad Sci U S A, vol. 85, no. 9, May 1988, pp. 3198–202. Pubmed, doi:10.1073/pnas.85.9.3198.
Rusche JR, Javaherian K, McDanal C, Petro J, Lynn DL, Grimaila R, Langlois A, Gallo RC, Arthur LO, Fischinger PJ. Antibodies that inhibit fusion of human immunodeficiency virus-infected cells bind a 24-amino acid sequence of the viral envelope, gp120. Proc Natl Acad Sci U S A. 1988 May;85(9):3198–3202.
Journal cover image

Published In

Proc Natl Acad Sci U S A

DOI

10.1073/pnas.85.9.3198

ISSN

0027-8424

Publication Date

May 1988

Volume

85

Issue

9

Start / End Page

3198 / 3202

Location

United States

Related Subject Headings

  • Viral Fusion Proteins
  • Retroviridae Proteins
  • Pan troglodytes
  • HIV Envelope Protein gp120
  • HIV
  • Epitopes
  • Enzyme-Linked Immunosorbent Assay
  • Blood
  • Animals
  • Amino Acid Sequence