Direct Conversion of Human Pluripotent Stem Cells to Osteoblasts With a Small Molecule.
Journal Article
Human pluripotent stem cells (hPSCs), which exhibit unlimited self-renewal ability and can differentiate into all cell types in the human body, are a promising cell source for cell-based therapies and regenerative medicine. Small molecules hold great potential in the derivation of tissue-specific cells from hPSCs owing to their cost-effectiveness and scalability. Here, we describe a protocol for deriving osteoblasts from hPSCs by using a single, natural small molecule: adenosine. This simple and effective experimental protocol allows one to obtain large numbers of osteoblasts or osteoprogenitor cells, with the ability to form functional bone tissues, from hPSCs, including human embryonic stem cells and induced pluripotent stem cells. This protocol could potentially enable studies of tissue regeneration and skeletal diseases. © 2018 by John Wiley & Sons, Inc.
Full Text
Duke Authors
Cited Authors
- Kang, H; Shih, Y-RV; Varghese, S
Published Date
- February 28, 2018
Published In
Volume / Issue
- 44 /
Start / End Page
- 1F.21.1 - 1F.21.6
PubMed ID
- 29512108
Pubmed Central ID
- 29512108
Electronic International Standard Serial Number (EISSN)
- 1938-8969
Digital Object Identifier (DOI)
- 10.1002/cpsc.44
Language
- eng
Conference Location
- United States