Modulation of microfilament protein composition by transfected cytoskeletal actin genes.
Journal Article (Journal Article)
HuT-14T is a highly tumorigenic fibroblast cell line which exhibits a reduced steady-state level of beta-actin due to coding mutations in one of two beta-actin alleles. The normal rate of total actin synthesis could be restored in some clones of cells following transfection of the functional beta-actin gene but not following transfection of the functional gamma-actin gene. In gamma-actin gene-transfected substrains that have increased rates of gamma-actin synthesis, beta-actin synthesis is further reduced in a manner consistent with an autoregulatory mechanism, resulting in abnormal ratios of actin isoforms. Thus, both beta- and gamma-actin proteins can apparently regulate the synthesis of their coexpressed isoforms. In addition, decreased synthesis of normal beta-actin seems to correlate with a concomitant down-regulation of tropomyosin isoforms.
Full Text
Duke Authors
Cited Authors
- Ng, SY; Erba, H; Latter, G; Kedes, L; Leavitt, J
Published Date
- April 1, 1988
Published In
Volume / Issue
- 8 / 4
Start / End Page
- 1790 - 1794
PubMed ID
- 3380097
Pubmed Central ID
- PMC363339
International Standard Serial Number (ISSN)
- 0270-7306
Digital Object Identifier (DOI)
- 10.1128/mcb.8.4.1790-1794.1988
Language
- eng
Conference Location
- United States