Modulation of microfilament protein composition by transfected cytoskeletal actin genes.
HuT-14T is a highly tumorigenic fibroblast cell line which exhibits a reduced steady-state level of beta-actin due to coding mutations in one of two beta-actin alleles. The normal rate of total actin synthesis could be restored in some clones of cells following transfection of the functional beta-actin gene but not following transfection of the functional gamma-actin gene. In gamma-actin gene-transfected substrains that have increased rates of gamma-actin synthesis, beta-actin synthesis is further reduced in a manner consistent with an autoregulatory mechanism, resulting in abnormal ratios of actin isoforms. Thus, both beta- and gamma-actin proteins can apparently regulate the synthesis of their coexpressed isoforms. In addition, decreased synthesis of normal beta-actin seems to correlate with a concomitant down-regulation of tropomyosin isoforms.
Duke Scholars
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- Tropomyosin
- Transfection
- Mutation
- Microfilament Proteins
- Humans
- Genes
- Gene Expression Regulation
- Developmental Biology
- Cytoskeleton
- Cell Line
Citation
Published In
DOI
ISSN
Publication Date
Volume
Issue
Start / End Page
Location
Related Subject Headings
- Tropomyosin
- Transfection
- Mutation
- Microfilament Proteins
- Humans
- Genes
- Gene Expression Regulation
- Developmental Biology
- Cytoskeleton
- Cell Line