Modulation of microfilament protein composition by transfected cytoskeletal actin genes.

Journal Article (Journal Article)

HuT-14T is a highly tumorigenic fibroblast cell line which exhibits a reduced steady-state level of beta-actin due to coding mutations in one of two beta-actin alleles. The normal rate of total actin synthesis could be restored in some clones of cells following transfection of the functional beta-actin gene but not following transfection of the functional gamma-actin gene. In gamma-actin gene-transfected substrains that have increased rates of gamma-actin synthesis, beta-actin synthesis is further reduced in a manner consistent with an autoregulatory mechanism, resulting in abnormal ratios of actin isoforms. Thus, both beta- and gamma-actin proteins can apparently regulate the synthesis of their coexpressed isoforms. In addition, decreased synthesis of normal beta-actin seems to correlate with a concomitant down-regulation of tropomyosin isoforms.

Full Text

Duke Authors

Cited Authors

  • Ng, SY; Erba, H; Latter, G; Kedes, L; Leavitt, J

Published Date

  • April 1, 1988

Published In

Volume / Issue

  • 8 / 4

Start / End Page

  • 1790 - 1794

PubMed ID

  • 3380097

Pubmed Central ID

  • PMC363339

International Standard Serial Number (ISSN)

  • 0270-7306

Digital Object Identifier (DOI)

  • 10.1128/mcb.8.4.1790-1794.1988


  • eng

Conference Location

  • United States