Salmonella Activation of STAT3 Signaling by SarA Effector Promotes Intracellular Replication and Production of IL-10.

Journal Article (Journal Article)

Salmonella enterica is an important foodborne pathogen that uses secreted effector proteins to manipulate host pathways to facilitate survival and dissemination. Different S. enterica serovars cause disease syndromes ranging from gastroenteritis to typhoid fever and vary in their effector repertoire. We leveraged this natural diversity to identify stm2585, here designated sarA (Salmonella anti-inflammatory response activator), as a Salmonella effector that induces production of the anti-inflammatory cytokine IL-10. RNA-seq of cells infected with either ΔsarA or wild-type S. Typhimurium revealed that SarA activates STAT3 transcriptional targets. Consistent with this, SarA is necessary and sufficient for STAT3 phosphorylation, STAT3 inhibition blocks IL-10 production, and SarA and STAT3 interact by co-immunoprecipitation. These effects of SarA contribute to intracellular replication in vitro and bacterial load at systemic sites in mice. Our results demonstrate the power of using comparative genomics for identifying effectors and that Salmonella has evolved mechanisms for activating an important anti-inflammatory pathway.

Full Text

Duke Authors

Cited Authors

  • Jaslow, SL; Gibbs, KD; Fricke, WF; Wang, L; Pittman, KJ; Mammel, MK; Thaden, JT; Fowler, VG; Hammer, GE; Elfenbein, JR; Ko, DC

Published Date

  • June 19, 2018

Published In

Volume / Issue

  • 23 / 12

Start / End Page

  • 3525 - 3536

PubMed ID

  • 29924996

Pubmed Central ID

  • PMC6314477

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2018.05.072


  • eng

Conference Location

  • United States