Real-time observation of leukocyte-endothelium interactions in tissue-engineered blood vessel.

Published

Journal Article

Human cell-based 3D tissue constructs play an increasing role in disease modeling and drug screening. Inflammation, atherosclerosis, and many autoimmune disorders involve the interactions between immune cells and blood vessels. However, it has been difficult to image and model these interactions under realistic conditions. In this study, we fabricated a perfusion and imaging chamber to allow the real-time visualization of leukocyte perfusion, adhesion, and migration inside a tissue-engineered blood vessel (TEBV). We monitored the elevated monocyte adhesion to the TEBV wall and transendothelial migration (TEM) as the TEBV endothelium was activated by the inflammatory cytokine TNF-α. We demonstrated that treatment with anti-TNF-α or an NF-kB signaling pathway inhibitor would attenuate the endothelium activation and reduce the number of leukocyte adhesion (>74%) and TEM events (>87%) close to the control. As the first demonstration of real-time imaging of dynamic cellular events within a TEBV, this work paves the way for drug screening and disease modeling in TEBV-associated microphysiological systems.

Full Text

Duke Authors

Cited Authors

  • Chen, Z; Tang, M; Huang, D; Jiang, W; Li, M; Ji, H; Park, J; Xu, B; Atchison, LJ; Truskey, GA; Leong, KW

Published Date

  • July 2018

Published In

Volume / Issue

  • 18 / 14

Start / End Page

  • 2047 - 2054

PubMed ID

  • 29927449

Pubmed Central ID

  • 29927449

Electronic International Standard Serial Number (EISSN)

  • 1473-0189

International Standard Serial Number (ISSN)

  • 1473-0197

Digital Object Identifier (DOI)

  • 10.1039/c8lc00202a

Language

  • eng