Intraamniotic Zika virus inoculation of pregnant rhesus macaques produces fetal neurologic disease.

Published online

Journal Article

Zika virus (ZIKV) infection of pregnant women can cause fetal microcephaly and other neurologic defects. We describe the development of a non-human primate model to better understand fetal pathogenesis. To reliably induce fetal infection at defined times, four pregnant rhesus macaques are inoculated intravenously and intraamniotically with ZIKV at gestational day (GD) 41, 50, 64, or 90, corresponding to first and second trimester of gestation. The GD41-inoculated animal, experiencing fetal death 7 days later, has high virus levels in fetal and placental tissues, implicating ZIKV as cause of death. The other three fetuses are carried to near term and euthanized; while none display gross microcephaly, all show ZIKV RNA in many tissues, especially in the brain, which exhibits calcifications and reduced neural precursor cells. Given that this model consistently recapitulates neurologic defects of human congenital Zika syndrome, it is highly relevant to unravel determinants of fetal neuropathogenesis and to explore interventions.

Full Text

Duke Authors

Cited Authors

  • Coffey, LL; Keesler, RI; Pesavento, PA; Woolard, K; Singapuri, A; Watanabe, J; Cruzen, C; Christe, KL; Usachenko, J; Yee, J; Heng, VA; Bliss-Moreau, E; Reader, JR; von Morgenland, W; Gibbons, AM; Jackson, K; Ardeshir, A; Heimsath, H; Permar, S; Senthamaraikannan, P; Presicce, P; Kallapur, SG; Linnen, JM; Gao, K; Orr, R; MacGill, T; McClure, M; McFarland, R; Morrison, JH; Van Rompay, KKA

Published Date

  • June 20, 2018

Published In

Volume / Issue

  • 9 / 1

Start / End Page

  • 2414 -

PubMed ID

  • 29925843

Pubmed Central ID

  • 29925843

Electronic International Standard Serial Number (EISSN)

  • 2041-1723

Digital Object Identifier (DOI)

  • 10.1038/s41467-018-04777-6

Language

  • eng

Conference Location

  • England