Molecular and biophysical analysis of apoptosis using a combined quantitative phase imaging and fluorescence resonance energy transfer microscope.

Published

Journal Article

Apoptotic mechanisms are often dysregulated in cancerous phenotypes. Additionally, many anticancer treatments induce apoptosis and necrosis, and the monitoring of this apoptotic activity can allow researchers to identify therapeutic efficiency. Here, we introduce a microscope which combines quantitative phase imaging (QPI) with the ability to detect molecular events via fluorescence (or Förster) resonance energy transfer (FRET). The system was applied to study cells undergoing apoptosis to correlate the onset of apoptotic enzyme activity as observed using a FRET-based apoptosis sensor with whole cell morphological changes analyzed via QPI. The QPI data showed changes in cell disorder strength during the initiation of apoptotic enzymatic activity.

Full Text

Duke Authors

Cited Authors

  • Eldridge, WJ; Hoballah, J; Wax, A

Published Date

  • December 2018

Published In

Volume / Issue

  • 11 / 12

Start / End Page

  • e201800126 -

PubMed ID

  • 29896886

Pubmed Central ID

  • 29896886

Electronic International Standard Serial Number (EISSN)

  • 1864-0648

International Standard Serial Number (ISSN)

  • 1864-063X

Digital Object Identifier (DOI)

  • 10.1002/jbio.201800126

Language

  • eng