Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro.
Journal Article (Journal Article)
Autophagy is an evolutionarily conserved intracellular catabolic process that is essential for cellular housekeeping and nutrient homeostasis. Recently, we provided evidence that thyroid hormone (TH) is a major inducer of autophagy in mammalian cells. Here, we describe a method for detecting TH-induced autophagic flux in hepatic, muscle, and brown adipocyte cells using lysosomal inhibitor bafilomycin A1 (BafA1) and conventional Western blot techniques.
Full Text
Duke Authors
Cited Authors
- Zhou, J; Sinha, RA; Lesmana, R; Yau, WWY; Yen, PM
Published Date
- 2018
Published In
Volume / Issue
- 1801 /
Start / End Page
- 111 - 122
PubMed ID
- 29892821
Pubmed Central ID
- PMC6020991
Electronic International Standard Serial Number (EISSN)
- 1940-6029
Digital Object Identifier (DOI)
- 10.1007/978-1-4939-7902-8_11
Language
- eng
Conference Location
- United States