Pharmacological Inhibition of Lysosomal Activity as a Method For Monitoring Thyroid Hormone-induced Autophagic Flux in Mammalian Cells In Vitro.

Journal Article (Journal Article)

Autophagy is an evolutionarily conserved intracellular catabolic process that is essential for cellular housekeeping and nutrient homeostasis. Recently, we provided evidence that thyroid hormone (TH) is a major inducer of autophagy in mammalian cells. Here, we describe a method for detecting TH-induced autophagic flux in hepatic, muscle, and brown adipocyte cells using lysosomal inhibitor bafilomycin A1 (BafA1) and conventional Western blot techniques.

Full Text

Duke Authors

Cited Authors

  • Zhou, J; Sinha, RA; Lesmana, R; Yau, WWY; Yen, PM

Published Date

  • 2018

Published In

Volume / Issue

  • 1801 /

Start / End Page

  • 111 - 122

PubMed ID

  • 29892821

Pubmed Central ID

  • PMC6020991

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-7902-8_11

Language

  • eng

Conference Location

  • United States