Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry.
Publication
, Journal Article
Popescu, I; Pipeling, M; Akulian, J; McDyer, J
Published in: Methods Mol Biol
2014
Cytotoxic T lymphocytes (CTLs) are important constituents of the adaptive immune system. Development of CTLs are particularly important for bacterial and viral infections, in addition to tumor surveillance. Measuring T cell immune function is important in evaluating host defense, allergy, autoimmunity, transplant rejection, and tumor immunity. In these recent years it has become possible to measure multiple effector functions in a single cell such as cytokine, transcription factors, and cytolytic function. In addition these parameters can be evaluated in conjunction with cellular proliferation. In this chapter we detail these cellular based assays and the methods used to characterize and quantify both phenotype and function of CTL.
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Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2014
Volume
1186
Start / End Page
21 / 47
Location
United States
Related Subject Headings
- fas Receptor
- T-Lymphocytes, Helper-Inducer
- T-Lymphocytes, Cytotoxic
- Staining and Labeling
- Receptors, Antigen, T-Cell
- Perforin
- Microspheres
- Lysosomal-Associated Membrane Protein 1
- Lymphocyte Activation
- Leukocyte Common Antigens
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Popescu, I., Pipeling, M., Akulian, J., & McDyer, J. (2014). Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry. Methods Mol Biol, 1186, 21–47. https://doi.org/10.1007/978-1-4939-1158-5_3
Popescu, Iulia, Matthew Pipeling, Jason Akulian, and John McDyer. “Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry.” Methods Mol Biol 1186 (2014): 21–47. https://doi.org/10.1007/978-1-4939-1158-5_3.
Popescu I, Pipeling M, Akulian J, McDyer J. Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry. Methods Mol Biol. 2014;1186:21–47.
Popescu, Iulia, et al. “Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry.” Methods Mol Biol, vol. 1186, 2014, pp. 21–47. Pubmed, doi:10.1007/978-1-4939-1158-5_3.
Popescu I, Pipeling M, Akulian J, McDyer J. Phenotypic and functional characterization of cytotoxic T lymphocytes by flow cytometry. Methods Mol Biol. 2014;1186:21–47.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2014
Volume
1186
Start / End Page
21 / 47
Location
United States
Related Subject Headings
- fas Receptor
- T-Lymphocytes, Helper-Inducer
- T-Lymphocytes, Cytotoxic
- Staining and Labeling
- Receptors, Antigen, T-Cell
- Perforin
- Microspheres
- Lysosomal-Associated Membrane Protein 1
- Lymphocyte Activation
- Leukocyte Common Antigens