In Vitro and In Vivo IgE-/Antigen-Mediated Mast Cell Activation.
Publication
, Journal Article
Choi, HW; Abraham, SN
Published in: Methods Mol Biol
2018
Mast cells (MCs) are major effectors of IgE-mediated allergic reactions because of their unique peripheral location and their powerful capacity to release prestored and de novo-synthesized inflammatory mediators into the circulation upon activation. In view of the growing incidence of allergy worldwide, there is much interest in developing novel strategies to block or temper IgE-mediated MC activation and its pathological consequences. For these studies, standard assays to measure IgE-mediated MC degranulation and mediator release are required. Here, we present detailed procedures to assess in vitro and in vivo MC release of prestored as well as recently synthesized mediators following IgE-/antigen-mediated activation.
Duke Scholars
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2018
Volume
1799
Start / End Page
71 / 80
Location
United States
Related Subject Headings
- Signal Transduction
- Mice
- Mast Cells
- Immunoglobulin E
- Developmental Biology
- Cell Degranulation
- Bone Marrow Cells
- Antigens
- Animals
- Anaphylaxis
Citation
APA
Chicago
ICMJE
MLA
NLM
Choi, H. W., & Abraham, S. N. (2018). In Vitro and In Vivo IgE-/Antigen-Mediated Mast Cell Activation. Methods Mol Biol, 1799, 71–80. https://doi.org/10.1007/978-1-4939-7896-0_7
Choi, Hae Woong, and Soman N. Abraham. “In Vitro and In Vivo IgE-/Antigen-Mediated Mast Cell Activation.” Methods Mol Biol 1799 (2018): 71–80. https://doi.org/10.1007/978-1-4939-7896-0_7.
Choi HW, Abraham SN. In Vitro and In Vivo IgE-/Antigen-Mediated Mast Cell Activation. Methods Mol Biol. 2018;1799:71–80.
Choi, Hae Woong, and Soman N. Abraham. “In Vitro and In Vivo IgE-/Antigen-Mediated Mast Cell Activation.” Methods Mol Biol, vol. 1799, 2018, pp. 71–80. Pubmed, doi:10.1007/978-1-4939-7896-0_7.
Choi HW, Abraham SN. In Vitro and In Vivo IgE-/Antigen-Mediated Mast Cell Activation. Methods Mol Biol. 2018;1799:71–80.
Published In
Methods Mol Biol
DOI
EISSN
1940-6029
Publication Date
2018
Volume
1799
Start / End Page
71 / 80
Location
United States
Related Subject Headings
- Signal Transduction
- Mice
- Mast Cells
- Immunoglobulin E
- Developmental Biology
- Cell Degranulation
- Bone Marrow Cells
- Antigens
- Animals
- Anaphylaxis