AFM-Based Single-Molecule Force Spectroscopy of Proteins.

Published

Journal Article

Single-molecule force spectroscopy by AFM (AFM-SMFS) is an experimental methodology that allows unequivocal sensitivity and control for investigating and manipulating the mechanical properties of single molecules. The past 20 years of AFM-SMFS has provided numerous breakthroughs in the understanding of the mechanical properties and force-induced structural rearrangements of sugars, DNA, and proteins. Here, we focus on the application of AFM-SMFS to study proteins, since AFM-SMFS has succeeded in providing abundant information about protein folding pathways, kinetics, interactions, and misfolding. In this chapter we describe the experimental procedures for conducting a SMFS-AFM experiment-including purification of protein samples, setup and calibration of the AFM instrumentation, and the thorough and unbiased analysis of resulting AFM data.

Full Text

Duke Authors

Cited Authors

  • Scholl, ZN; Marszalek, PE

Published Date

  • January 2018

Published In

Volume / Issue

  • 1814 /

Start / End Page

  • 35 - 47

PubMed ID

  • 29956225

Pubmed Central ID

  • 29956225

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

International Standard Serial Number (ISSN)

  • 1064-3745

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-8591-3_3

Language

  • eng