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AFM-Based Single-Molecule Force Spectroscopy of Proteins.

Publication ,  Journal Article
Scholl, ZN; Marszalek, PE
Published in: Methods in molecular biology (Clifton, N.J.)
January 2018

Single-molecule force spectroscopy by AFM (AFM-SMFS) is an experimental methodology that allows unequivocal sensitivity and control for investigating and manipulating the mechanical properties of single molecules. The past 20 years of AFM-SMFS has provided numerous breakthroughs in the understanding of the mechanical properties and force-induced structural rearrangements of sugars, DNA, and proteins. Here, we focus on the application of AFM-SMFS to study proteins, since AFM-SMFS has succeeded in providing abundant information about protein folding pathways, kinetics, interactions, and misfolding. In this chapter we describe the experimental procedures for conducting a SMFS-AFM experiment-including purification of protein samples, setup and calibration of the AFM instrumentation, and the thorough and unbiased analysis of resulting AFM data.

Duke Scholars

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2018

Volume

1814

Start / End Page

35 / 47

Related Subject Headings

  • Proteins
  • Protein Unfolding
  • Microscopy, Atomic Force
  • Humans
  • Developmental Biology
  • Data Analysis
  • Cell Line
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology
 

Citation

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Chicago
ICMJE
MLA
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Scholl, Z. N., & Marszalek, P. E. (2018). AFM-Based Single-Molecule Force Spectroscopy of Proteins. Methods in Molecular Biology (Clifton, N.J.), 1814, 35–47. https://doi.org/10.1007/978-1-4939-8591-3_3
Scholl, Zackary N., and Piotr E. Marszalek. “AFM-Based Single-Molecule Force Spectroscopy of Proteins.Methods in Molecular Biology (Clifton, N.J.) 1814 (January 2018): 35–47. https://doi.org/10.1007/978-1-4939-8591-3_3.
Scholl ZN, Marszalek PE. AFM-Based Single-Molecule Force Spectroscopy of Proteins. Methods in molecular biology (Clifton, NJ). 2018 Jan;1814:35–47.
Scholl, Zackary N., and Piotr E. Marszalek. “AFM-Based Single-Molecule Force Spectroscopy of Proteins.Methods in Molecular Biology (Clifton, N.J.), vol. 1814, Jan. 2018, pp. 35–47. Epmc, doi:10.1007/978-1-4939-8591-3_3.
Scholl ZN, Marszalek PE. AFM-Based Single-Molecule Force Spectroscopy of Proteins. Methods in molecular biology (Clifton, NJ). 2018 Jan;1814:35–47.

Published In

Methods in molecular biology (Clifton, N.J.)

DOI

EISSN

1940-6029

ISSN

1064-3745

Publication Date

January 2018

Volume

1814

Start / End Page

35 / 47

Related Subject Headings

  • Proteins
  • Protein Unfolding
  • Microscopy, Atomic Force
  • Humans
  • Developmental Biology
  • Data Analysis
  • Cell Line
  • Animals
  • 3404 Medicinal and biomolecular chemistry
  • 3101 Biochemistry and cell biology