HIV-1 envelope glycoprotein trimers display open quaternary conformation when bound to the gp41 membrane-proximal external-region-directed broadly neutralizing antibody Z13e1.

Journal Article (Journal Article)

We describe cryo-electron microscopic studies of the interaction between the ectodomain of the trimeric HIV-1 envelope glycoprotein (Env) and Z13e1, a broadly neutralizing antibody that targets the membrane-proximal external region (MPER) of the gp41 subunit. We show that Z13e1-bound Env displays an open quaternary conformation similar to the CD4-bound conformation. Our results support the idea that MPER-directed antibodies, such as Z13e1, block viral entry by interacting with Env at a step after CD4 activation.

Full Text

Duke Authors

Cited Authors

  • Harris, AK; Bartesaghi, A; Milne, JLS; Subramaniam, S

Published Date

  • June 2013

Published In

Volume / Issue

  • 87 / 12

Start / End Page

  • 7191 - 7196

PubMed ID

  • 23596305

Pubmed Central ID

  • PMC3676106

Electronic International Standard Serial Number (EISSN)

  • 1098-5514

International Standard Serial Number (ISSN)

  • 0022-538X

Digital Object Identifier (DOI)

  • 10.1128/jvi.03284-12


  • eng