Electron tomography of viruses.

Journal Article (Review;Journal Article)

Understanding the molecular architectures of enveloped and complex viruses is a challenging frontier in structural biology. In these viruses, the structural and compositional variation from one viral particle to another generally precludes the use of either crystallization or image averaging procedures that have been successfully implemented in the past for highly symmetric viruses. While advances in cryo electron tomography of unstained specimens provide new opportunities for identification and molecular averaging of individual subcomponents such as the surface glycoprotein spikes on purified viruses, electron tomography of stained and plunge-frozen cells is being used to visualize the cellular context of viral entry and replication. Here, we review recent developments in both areas as they relate to our understanding of the biology of heterogeneous and pleiomorphic viruses.

Full Text

Duke Authors

Cited Authors

  • Subramaniam, S; Bartesaghi, A; Liu, J; Bennett, AE; Sougrat, R

Published Date

  • October 2007

Published In

Volume / Issue

  • 17 / 5

Start / End Page

  • 596 - 602

PubMed ID

  • 17964134

Pubmed Central ID

  • PMC2121662

Electronic International Standard Serial Number (EISSN)

  • 1879-033X

International Standard Serial Number (ISSN)

  • 0959-440X

Digital Object Identifier (DOI)

  • 10.1016/j.sbi.2007.09.009


  • eng