Spotiton: New features and applications.

Journal Article (Journal Article)

We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ∼3 Å resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples.

Full Text

Duke Authors

Cited Authors

  • Dandey, VP; Wei, H; Zhang, Z; Tan, YZ; Acharya, P; Eng, ET; Rice, WJ; Kahn, PA; Potter, CS; Carragher, B

Published Date

  • May 2018

Published In

Volume / Issue

  • 202 / 2

Start / End Page

  • 161 - 169

PubMed ID

  • 29366716

Pubmed Central ID

  • PMC6317895

Electronic International Standard Serial Number (EISSN)

  • 1095-8657

Digital Object Identifier (DOI)

  • 10.1016/j.jsb.2018.01.002

Language

  • eng

Conference Location

  • United States