Spotiton: New features and applications.
Published
Journal Article
We present an update describing new features and applications of Spotiton, a novel instrument for vitrifying samples for cryoEM. We have used Spotiton to prepare several test specimens that can be reconstructed using routine single particle analysis to ∼3 Å resolution, indicating that the process has no apparent deleterious effect on the sample integrity. The system is now in routine and continuous use in our lab and has been used to successfully vitrify a wide variety of samples.
Full Text
Duke Authors
Cited Authors
- Dandey, VP; Wei, H; Zhang, Z; Tan, YZ; Acharya, P; Eng, ET; Rice, WJ; Kahn, PA; Potter, CS; Carragher, B
Published Date
- May 2018
Published In
Volume / Issue
- 202 / 2
Start / End Page
- 161 - 169
PubMed ID
- 29366716
Pubmed Central ID
- 29366716
Electronic International Standard Serial Number (EISSN)
- 1095-8657
Digital Object Identifier (DOI)
- 10.1016/j.jsb.2018.01.002
Language
- eng
Conference Location
- United States