Skip to main content
Journal cover image

Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4.

Publication ,  Journal Article
Huang, C-C; Lam, SN; Acharya, P; Tang, M; Xiang, S-H; Hussan, SS-U; Stanfield, RL; Robinson, J; Sodroski, J; Wilson, IA; Wyatt, R; Bewley, CA ...
Published in: Science
September 28, 2007

The CCR5 co-receptor binds to the HIV-1 gp120 envelope glycoprotein and facilitates HIV-1 entry into cells. Its N terminus is tyrosine-sulfated, as are many antibodies that react with the co-receptor binding site on gp120. We applied nuclear magnetic resonance and crystallographic techniques to analyze the structure of the CCR5 N terminus and that of the tyrosine-sulfated antibody 412d in complex with gp120 and CD4. The conformations of tyrosine-sulfated regions of CCR5 (alpha-helix) and 412d (extended loop) are surprisingly different. Nonetheless, a critical sulfotyrosine on CCR5 and on 412d induces similar structural rearrangements in gp120. These results now provide a framework for understanding HIV-1 interactions with the CCR5 N terminus during viral entry and define a conserved site on gp120, whose recognition of sulfotyrosine engenders posttranslational mimicry by the immune system.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

Science

DOI

EISSN

1095-9203

Publication Date

September 28, 2007

Volume

317

Issue

5846

Start / End Page

1930 / 1934

Location

United States

Related Subject Headings

  • Virus Internalization
  • Tyrosine
  • Sulfates
  • Receptors, CCR5
  • Peptide Fragments
  • Nuclear Magnetic Resonance, Biomolecular
  • Molecular Sequence Data
  • Molecular Mimicry
  • Models, Molecular
  • Humans
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Huang, C.-C., Lam, S. N., Acharya, P., Tang, M., Xiang, S.-H., Hussan, S.-U., … Kwong, P. D. (2007). Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4. Science, 317(5846), 1930–1934. https://doi.org/10.1126/science.1145373
Huang, Chih-Chin, Son N. Lam, Priyamvada Acharya, Min Tang, Shi-Hua Xiang, Syed Shahzad-Ul Hussan, Robyn L. Stanfield, et al. “Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4.Science 317, no. 5846 (September 28, 2007): 1930–34. https://doi.org/10.1126/science.1145373.
Huang C-C, Lam SN, Acharya P, Tang M, Xiang S-H, Hussan SS-U, et al. Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4. Science. 2007 Sep 28;317(5846):1930–4.
Huang, Chih-Chin, et al. “Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4.Science, vol. 317, no. 5846, Sept. 2007, pp. 1930–34. Pubmed, doi:10.1126/science.1145373.
Huang C-C, Lam SN, Acharya P, Tang M, Xiang S-H, Hussan SS-U, Stanfield RL, Robinson J, Sodroski J, Wilson IA, Wyatt R, Bewley CA, Kwong PD. Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4. Science. 2007 Sep 28;317(5846):1930–1934.
Journal cover image

Published In

Science

DOI

EISSN

1095-9203

Publication Date

September 28, 2007

Volume

317

Issue

5846

Start / End Page

1930 / 1934

Location

United States

Related Subject Headings

  • Virus Internalization
  • Tyrosine
  • Sulfates
  • Receptors, CCR5
  • Peptide Fragments
  • Nuclear Magnetic Resonance, Biomolecular
  • Molecular Sequence Data
  • Molecular Mimicry
  • Models, Molecular
  • Humans