Structures of the CCR5 N terminus and of a tyrosine-sulfated antibody with HIV-1 gp120 and CD4.

Published

Journal Article

The CCR5 co-receptor binds to the HIV-1 gp120 envelope glycoprotein and facilitates HIV-1 entry into cells. Its N terminus is tyrosine-sulfated, as are many antibodies that react with the co-receptor binding site on gp120. We applied nuclear magnetic resonance and crystallographic techniques to analyze the structure of the CCR5 N terminus and that of the tyrosine-sulfated antibody 412d in complex with gp120 and CD4. The conformations of tyrosine-sulfated regions of CCR5 (alpha-helix) and 412d (extended loop) are surprisingly different. Nonetheless, a critical sulfotyrosine on CCR5 and on 412d induces similar structural rearrangements in gp120. These results now provide a framework for understanding HIV-1 interactions with the CCR5 N terminus during viral entry and define a conserved site on gp120, whose recognition of sulfotyrosine engenders posttranslational mimicry by the immune system.

Full Text

Duke Authors

Cited Authors

  • Huang, C-C; Lam, SN; Acharya, P; Tang, M; Xiang, S-H; Hussan, SS-U; Stanfield, RL; Robinson, J; Sodroski, J; Wilson, IA; Wyatt, R; Bewley, CA; Kwong, PD

Published Date

  • September 28, 2007

Published In

Volume / Issue

  • 317 / 5846

Start / End Page

  • 1930 - 1934

PubMed ID

  • 17901336

Pubmed Central ID

  • 17901336

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.1145373

Language

  • eng

Conference Location

  • United States