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Diffraction tomography with Fourier ptychography.

Publication ,  Journal Article
Horstmeyer, R; Chung, J; Ou, X; Zheng, G; Yang, C
Published in: Optica
August 2016

This paper presents a technique to image the complex index of refraction of a sample across three dimensions. The only required hardware is a standard microscope and an array of LEDs. The method, termed Fourier ptychographic tomography (FPT), first captures a sequence of intensity-only images of a sample under angularly varying illumination. Then, using principles from ptychography and diffraction tomography, it computationally solves for the sample structure in three dimensions. The experimental microscope demonstrates a lateral spatial resolution of 0.39 μm and an axial resolution of 3.7 μm at the Nyquist-Shannon sampling limit (0.54 and 5.0 μm at the Sparrow limit, respectively) across a total imaging depth of 110 μm. Unlike competing methods, this technique quantitatively measures the volumetric refractive index of primarily transparent and contiguous sample features without the need for interferometry or any moving parts. Wide field-of-view reconstructions of thick biological specimens suggest potential applications in pathology and developmental biology.

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Published In

Optica

DOI

EISSN

2334-2536

ISSN

2334-2536

Publication Date

August 2016

Volume

3

Issue

8

Start / End Page

827 / 835

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 1005 Communications Technologies
  • 0906 Electrical and Electronic Engineering
  • 0205 Optical Physics
 

Citation

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Horstmeyer, R., Chung, J., Ou, X., Zheng, G., & Yang, C. (2016). Diffraction tomography with Fourier ptychography. Optica, 3(8), 827–835. https://doi.org/10.1364/optica.3.000827
Horstmeyer, Roarke, Jaebum Chung, Xiaoze Ou, Guoan Zheng, and Changhuei Yang. “Diffraction tomography with Fourier ptychography.Optica 3, no. 8 (August 2016): 827–35. https://doi.org/10.1364/optica.3.000827.
Horstmeyer R, Chung J, Ou X, Zheng G, Yang C. Diffraction tomography with Fourier ptychography. Optica. 2016 Aug;3(8):827–35.
Horstmeyer, Roarke, et al. “Diffraction tomography with Fourier ptychography.Optica, vol. 3, no. 8, Aug. 2016, pp. 827–35. Epmc, doi:10.1364/optica.3.000827.
Horstmeyer R, Chung J, Ou X, Zheng G, Yang C. Diffraction tomography with Fourier ptychography. Optica. 2016 Aug;3(8):827–835.
Journal cover image

Published In

Optica

DOI

EISSN

2334-2536

ISSN

2334-2536

Publication Date

August 2016

Volume

3

Issue

8

Start / End Page

827 / 835

Related Subject Headings

  • 5102 Atomic, molecular and optical physics
  • 1005 Communications Technologies
  • 0906 Electrical and Electronic Engineering
  • 0205 Optical Physics