Digital pathology with Fourier ptychography.


Journal Article

Fourier ptychographic microscopy (FPM) is a recently introduced method of acquiring high-resolution, wide field of view (FOV) giga-pixel histology images. The FPM procedure first acquires a sequence of low-resolution images of a sample under variable-angle illumination. It then combines these images using a novel phase retrieval algorithm to improve the employed microscope's resolution beyond its conventional limit. Here, we first describe how FPM's resolution improvement can enhance wide FOV histology imaging. Second, we show that FPM also records a thin sample's optical phase, which can help pathologists digitally extract as much information as possible from a given histology slide.

Full Text

Duke Authors

Cited Authors

  • Horstmeyer, R; Ou, X; Zheng, G; Willems, P; Yang, C

Published Date

  • June 2015

Published In

Volume / Issue

  • 42 /

Start / End Page

  • 38 - 43

PubMed ID

  • 25481664

Pubmed Central ID

  • 25481664

Electronic International Standard Serial Number (EISSN)

  • 1879-0771

International Standard Serial Number (ISSN)

  • 0895-6111

Digital Object Identifier (DOI)

  • 10.1016/j.compmedimag.2014.11.005


  • eng