High numerical aperture Fourier ptychography: principle, implementation and characterization.

Journal Article (Journal Article)

Fourier ptychography (FP) utilizes illumination control and computational post-processing to increase the resolution of bright-field microscopes. In effect, FP extends the fixed numerical aperture (NA) of an objective lens to form a larger synthetic system NA. Here, we build an FP microscope (FPM) using a 40X 0.75NA objective lens to synthesize a system NA of 1.45. This system achieved a two-slit resolution of 335 nm at a wavelength of 632 nm. This resolution closely adheres to theoretical prediction and is comparable to the measured resolution (315 nm) associated with a standard, commercially available 1.25 NA oil immersion microscope. Our work indicates that Fourier ptychography is an attractive method to improve the resolution-versus-NA performance, increase the working distance, and enlarge the field-of-view of high-resolution bright-field microscopes by employing lower NA objectives.

Full Text

Duke Authors

Cited Authors

  • Ou, X; Horstmeyer, R; Zheng, G; Yang, C

Published Date

  • February 2015

Published In

Volume / Issue

  • 23 / 3

Start / End Page

  • 3472 - 3491

PubMed ID

  • 25836203

Pubmed Central ID

  • PMC5802253

Electronic International Standard Serial Number (EISSN)

  • 1094-4087

International Standard Serial Number (ISSN)

  • 1094-4087

Digital Object Identifier (DOI)

  • 10.1364/oe.23.003472


  • eng