High-depth transcriptomic profiling reveals the temporal gene signature of human mesenchymal stem cells during chondrogenesis.
Mesenchymal stem/stromal cells (MSCs) provide an attractive cell source for cartilage repair and cell therapy; however, the underlying molecular pathways that drive chondrogenesis of these populations of adult stem cells remain poorly understood. We generated a rich data set of high-throughput RNA sequencing of human MSCs throughout chondrogenesis at 6 different time points. Our data consisted of 18 libraries with 3 individual donors as biologic replicates, with each library possessing a sequencing depth of 100 million reads. Computational analyses with differential gene expression, gene ontology, and weighted gene correlation network analysis identified dynamic changes in multiple biologic pathways and, most importantly, a chondrogenic gene subset, whose functional characterization promises to further harness the potential of MSCs for cartilage tissue engineering. Furthermore, we created a graphic user interface encyclopedia built with the goal of producing an open resource of transcriptomic regulation for additional data mining and pathway analysis of the process of MSC chondrogenesis.-Huynh, N. P. T., Zhang, B., Guilak, F. High-depth transcriptomic profiling reveals the temporal gene signature of human mesenchymal stem cells during chondrogenesis.
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Related Subject Headings
- Tissue Engineering
- Signal Transduction
- Mesenchymal Stem Cells
- Humans
- High-Throughput Nucleotide Sequencing
- Gene Regulatory Networks
- Gene Expression Profiling
- Chondrocytes
- Cells, Cultured
- Cell Differentiation
Citation
Published In
DOI
EISSN
ISSN
Publication Date
Volume
Issue
Start / End Page
Related Subject Headings
- Tissue Engineering
- Signal Transduction
- Mesenchymal Stem Cells
- Humans
- High-Throughput Nucleotide Sequencing
- Gene Regulatory Networks
- Gene Expression Profiling
- Chondrocytes
- Cells, Cultured
- Cell Differentiation