GC content elevates mutation and recombination rates in the yeast Saccharomyces cerevisiae.


Journal Article

The chromosomes of many eukaryotes have regions of high GC content interspersed with regions of low GC content. In the yeast Saccharomyces cerevisiae, high-GC regions are often associated with high levels of meiotic recombination. In this study, we constructed URA3 genes that differ substantially in their base composition [URA3-AT (31% GC), URA3-WT (43% GC), and URA3-GC (63% GC)] but encode proteins with the same amino acid sequence. The strain with URA3-GC had an approximately sevenfold elevated rate of ura3 mutations compared with the strains with URA3-WT or URA3-AT About half of these mutations were single-base substitutions and were dependent on the error-prone DNA polymerase ζ. About 30% were deletions or duplications between short (5-10 base) direct repeats resulting from DNA polymerase slippage. The URA3-GC gene also had elevated rates of meiotic and mitotic recombination relative to the URA3-AT or URA3-WT genes. Thus, base composition has a substantial effect on the basic parameters of genome stability and evolution.

Full Text

Duke Authors

Cited Authors

  • Kiktev, DA; Sheng, Z; Lobachev, KS; Petes, TD

Published Date

  • July 24, 2018

Published In

Volume / Issue

  • 115 / 30

Start / End Page

  • E7109 - E7118

PubMed ID

  • 29987035

Pubmed Central ID

  • 29987035

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Digital Object Identifier (DOI)

  • 10.1073/pnas.1807334115


  • eng

Conference Location

  • United States