Isolation and Characterization of Human Lung Myeloid Cells.
Published
Journal Article
Multiparameter flow cytometry of human lungs allows for characterization, isolation, and examination of human pulmonary immune cell composition, phenotype, and function. Here we describe an approach to process lung tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This base antibody panel can also be used to identify major immune cell types in human blood and bronchoalveolar lavage (BAL) fluid.
Full Text
Duke Authors
Cited Authors
- Yu, Y-RA; Tighe, RM
Published Date
- 2018
Published In
Volume / Issue
- 1809 /
Start / End Page
- 111 - 119
PubMed ID
- 29987786
Pubmed Central ID
- 29987786
Electronic International Standard Serial Number (EISSN)
- 1940-6029
Digital Object Identifier (DOI)
- 10.1007/978-1-4939-8570-8_9
Language
- eng
Conference Location
- United States