Isolation and Characterization of Human Lung Myeloid Cells.

Journal Article (Journal Article)

Multiparameter flow cytometry of human lungs allows for characterization, isolation, and examination of human pulmonary immune cell composition, phenotype, and function. Here we describe an approach to process lung tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This base antibody panel can also be used to identify major immune cell types in human blood and bronchoalveolar lavage (BAL) fluid.

Full Text

Duke Authors

Cited Authors

  • Yu, Y-RA; Tighe, RM

Published Date

  • 2018

Published In

Volume / Issue

  • 1809 /

Start / End Page

  • 111 - 119

PubMed ID

  • 29987786

Pubmed Central ID

  • 29987786

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Digital Object Identifier (DOI)

  • 10.1007/978-1-4939-8570-8_9

Language

  • eng

Conference Location

  • United States