Isolation and Characterization of Human Lung Myeloid Cells.

Published

Journal Article

Multiparameter flow cytometry of human lungs allows for characterization, isolation, and examination of human pulmonary immune cell composition, phenotype, and function. Here we describe an approach to process lung tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This base antibody panel can also be used to identify major immune cell types in human blood and bronchoalveolar lavage (BAL) fluid.

Full Text

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Duke Authors

Tighe, Robert Matthew
Yu, Yen-Rei Andrea

Cited Authors

Yu, Y-RA; Tighe, RM

Published Date

January 2018

Published In

Methods in Molecular Biology (Clifton, N.J.)

Volume / Issue

1809 /

Start / End Page

111 - 119

PubMed ID

29987786

Pubmed Central ID

29987786

Electronic International Standard Serial Number (EISSN)

1940-6029

International Standard Serial Number (ISSN)

1064-3745

Digital Object Identifier (DOI)

10.1007/978-1-4939-8570-8_9

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