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Isolation and Characterization of Human Lung Myeloid Cells.

Publication ,  Journal Article
Yu, Y-RA; Tighe, RM
Published in: Methods Mol Biol
2018

Multiparameter flow cytometry of human lungs allows for characterization, isolation, and examination of human pulmonary immune cell composition, phenotype, and function. Here we describe an approach to process lung tissues and then utilize a base antibody panel to define all of the major immune cell types in a single staining condition. This base antibody panel can also be used to identify major immune cell types in human blood and bronchoalveolar lavage (BAL) fluid.

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Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2018

Volume

1809

Start / End Page

111 / 119

Location

United States

Related Subject Headings

  • Myeloid Cells
  • Monocytes
  • Macrophages, Alveolar
  • Lung
  • Immunophenotyping
  • Humans
  • Flow Cytometry
  • Developmental Biology
  • Cell Survival
  • Cell Separation
 

Citation

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Yu, Y.-R., & Tighe, R. M. (2018). Isolation and Characterization of Human Lung Myeloid Cells. Methods Mol Biol, 1809, 111–119. https://doi.org/10.1007/978-1-4939-8570-8_9
Yu, Yen-Rei A., and Robert M. Tighe. “Isolation and Characterization of Human Lung Myeloid Cells.Methods Mol Biol 1809 (2018): 111–19. https://doi.org/10.1007/978-1-4939-8570-8_9.
Yu Y-RA, Tighe RM. Isolation and Characterization of Human Lung Myeloid Cells. Methods Mol Biol. 2018;1809:111–9.
Yu, Yen-Rei A., and Robert M. Tighe. “Isolation and Characterization of Human Lung Myeloid Cells.Methods Mol Biol, vol. 1809, 2018, pp. 111–19. Pubmed, doi:10.1007/978-1-4939-8570-8_9.
Yu Y-RA, Tighe RM. Isolation and Characterization of Human Lung Myeloid Cells. Methods Mol Biol. 2018;1809:111–119.

Published In

Methods Mol Biol

DOI

EISSN

1940-6029

Publication Date

2018

Volume

1809

Start / End Page

111 / 119

Location

United States

Related Subject Headings

  • Myeloid Cells
  • Monocytes
  • Macrophages, Alveolar
  • Lung
  • Immunophenotyping
  • Humans
  • Flow Cytometry
  • Developmental Biology
  • Cell Survival
  • Cell Separation