Cryo-EM structure of a mitochondrial calcium uniporter.

Journal Article (Journal Article)

Calcium transport plays an important role in regulating mitochondrial physiology and pathophysiology. The mitochondrial calcium uniporter (MCU) is a calcium-selective ion channel that is the primary mediator for calcium uptake into the mitochondrial matrix. Here, we present the cryo-electron microscopy structure of the full-length MCU from Neurospora crassa to an overall resolution of ~3.7 angstroms. Our structure reveals a tetrameric architecture, with the soluble and transmembrane domains adopting different symmetric arrangements within the channel. The conserved W-D-Φ-Φ-E-P-V-T-Y sequence motif of MCU pore forms a selectivity filter comprising two acidic rings separated by one helical turn along the central axis of the channel pore. The structure combined with mutagenesis gives insight into the basis of calcium recognition.

Full Text

Duke Authors

Cited Authors

  • Yoo, J; Wu, M; Yin, Y; Herzik, MA; Lander, GC; Lee, S-Y

Published Date

  • August 3, 2018

Published In

Volume / Issue

  • 361 / 6401

Start / End Page

  • 506 - 511

PubMed ID

  • 29954988

Pubmed Central ID

  • PMC6155975

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Digital Object Identifier (DOI)

  • 10.1126/science.aar4056


  • eng

Conference Location

  • United States