Substance P and calcitonin gene-related peptide increase IL-1 beta, IL-6 and TNF alpha secretion from human peripheral blood mononuclear cells.

Journal Article (Journal Article)

We found that substance P (SP) and calcitonin gene-related peptide (CGRP) (0.3-1 microM) increased, in a concentration-dependent manner, the basal secretion of interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF alpha) from cultured lymphocyte-enriched mononuclear cells isolated from human peripheral blood. SP and CGRP (0.1 microM) synergistically increased basal TNF alpha secretion. Dynorphin A((1-17)) (0.1-1 microM) did not modify basal cytokine secretion. Lipopolysaccharide (10 ng/ml)-induced cytokine secretion and [(3)H]thymidine uptake were not altered by any neuropeptide (at 0.1 microM). Thus, SP and CGRP stimulate the production of pro-inflammatory cytokines from lymphocytes only at high concentrations, similar to those reached during tissue damage.

Full Text

Duke Authors

Cited Authors

  • Cuesta, MC; Quintero, L; Pons, H; Suarez-Roca, H

Published Date

  • April 2002

Published In

Volume / Issue

  • 40 / 4

Start / End Page

  • 301 - 306

PubMed ID

  • 11792459

Electronic International Standard Serial Number (EISSN)

  • 1872-9754

International Standard Serial Number (ISSN)

  • 0197-0186

Digital Object Identifier (DOI)

  • 10.1016/s0197-0186(01)00094-8


  • eng