Chemical composition and biological activity of extracts from arrabidaea bilabiata.

Journal Article (Journal Article)

Consumption of Arrabidaea bilabiata fresh leaves produces a paraplegic syndrome in cattle. For isolation of the active principle, isopropanol and methanol extracts were prepared from the aerial parts of the plant; these were administered to Sprague-Dawley rats at doses of 0.5, 1, and 2 mg/kg, orally, to determine the presence of substances affecting spontaneous motor activity. The isopropanol extract produced significant increases of long-pause and short-pause movements (112 and 54%, respectively). The methanol extract tended to increase motor activity, but the effect did not reach statistical significance. Chemical and spectroscopic analysis of the isopropanol extract showed allantoin was the major constituent. It was administered to rats, at doses of 1.2, 12, and 120 mg/kg. A complex dose-response curve was observed, but at the highest dose, there was a 52% increase in the number of long-pause movements. It was inferred that allantoin and the constituents of the alcholic extracts might not be responsible for the paraplegic synndrome in cattle. The alteration of cattle motor activity could be caused by other non-alcoholic constituents. Additionally, extracts were assayed for in vitro activity against Escherichia coli , Klebsiella pneumonae , Pseudomonas aeruginosa , Staphylococcus aureus , Bacillus sp. and Candida albicans . All microbial strains were found to be resistant to the extracts with the exception of C. albicans , suggesting a possible antifungal activity.

Full Text

Duke Authors

Cited Authors

  • González, B; Suárez-Roca, H; Bravo, A; Salas-Auvert, R; Avila, D

Published Date

  • January 2000

Published In

Volume / Issue

  • 38 / 4

Start / End Page

  • 287 - 290

PubMed ID

  • 21214478

Electronic International Standard Serial Number (EISSN)

  • 1744-5116

International Standard Serial Number (ISSN)

  • 1388-0209

Digital Object Identifier (DOI)

  • 10.1076/1388-0209(200009)3841-aft287


  • eng