Meningeal whole mount preparation and characterization of neural cells by flow cytometry.

Journal Article (Journal Article)

Neuroimmunologists aim to understand the interactions between the central nervous system and the immune system under both homeostatic and pathological conditions. The meninges, contrary to the brain parenchyma, are populated by numerous immune cells. Soluble factors produced by these cells are capable to diffuse into the brain parenchyma and influence the brain cells within the parenchyma, including neurons. In this unit, we will describe two protocols: analysis the meningeal compartment of rodents and the use flow cytometry to study the cells of the brain parenchyma (particularly neurons).

Full Text

Duke Authors

Cited Authors

  • Louveau, A; Filiano, AJ; Kipnis, J

Published Date

  • April 2018

Published In

Volume / Issue

  • 121 / 1

Start / End Page

  • e50 -

PubMed ID

  • 30008983

Pubmed Central ID

  • PMC6040815

Electronic International Standard Serial Number (EISSN)

  • 1934-368X

Digital Object Identifier (DOI)

  • 10.1002/cpim.50


  • eng

Conference Location

  • United States