Antiviral Effects of ABMA against Herpes Simplex Virus Type 2 In Vitro and In Vivo.

Published

Journal Article

Herpes simplex virus type 2 (HSV-2) is the causative pathogen of genital herpes and is closely associated with the occurrence of cervical cancer and human immunodeficiency virus (HIV) infection. The absence of an effective vaccine and the emergence of drug resistance to commonly used nucleoside analogs emphasize the urgent need for alternative antivirals against HSV-2. Recently, ABMA [1-adamantyl (5-bromo-2-methoxybenzyl) amine] has been demonstrated to be an inhibitor of several pathogens exploiting host-vesicle transport, which also participates in the HSV-2 lifecycle. Here, we showed that ABMA inhibited HSV-2-induced cytopathic effects and plaque formation with 50% effective concentrations of 1.66 and 1.08 μM, respectively. We also preliminarily demonstrated in a time of compound addition assay that ABMA exerted a dual antiviral mechanism by impairing virus entry, as well as the late stages of the HSV-2 lifecycle. Furthermore, in vivo studies showed that ABMA protected BALB/c mice from intravaginal HSV-2 challenge with an improved survival rate of 50% at 5 mg/kg (8.33% for the untreated virus infected control). Consequently, our study has identified ABMA as an effective inhibitor of HSV-2, both in vitro and in vivo, for the first time and presents an alternative to nucleoside analogs for HSV-2 infection treatment.

Full Text

Duke Authors

Cited Authors

  • Dai, W; Wu, Y; Bi, J; Wang, S; Li, F; Kong, W; Barbier, J; Cintrat, J-C; Gao, F; Gillet, D; Su, W; Jiang, C

Published Date

  • March 9, 2018

Published In

Volume / Issue

  • 10 / 3

PubMed ID

  • 29522484

Pubmed Central ID

  • 29522484

Electronic International Standard Serial Number (EISSN)

  • 1999-4915

International Standard Serial Number (ISSN)

  • 1999-4915

Digital Object Identifier (DOI)

  • 10.3390/v10030119

Language

  • eng