Skip to main content

Capturing change in clonal composition amongst single mouse germinal centers.

Publication ,  Journal Article
Firl, DJ; Degn, SE; Padera, T; Carroll, MC
Published in: eLife
August 2018

Understanding cellular processes occurring in vivo on time scales of days to weeks requires repeatedly interrogating the same tissue without perturbing homeostasis. We describe a novel setup for longitudinal intravital imaging of murine peripheral lymph nodes (LNs). The formation and evolution of single germinal centers (GCs) was visualized over days to weeks. Naïve B cells encounter antigen and form primary foci, which subsequently seed GCs. These experience widely varying rates of homogenizing selection, even within closely confined spatial proximity. The fluidity of GCs is greater than previously observed with large shifts in clonality over short time scales; and loss of GCs is a rare, observable event. The observation of contemporaneous, congruent shifts in clonal composition between GCs within the same animal suggests inter-GC trafficking of memory B cells. This tool refines approaches to resolving immune dynamics in peripheral LNs with high temporospatial resolution and minimal perturbation of homeostasis.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

eLife

DOI

EISSN

2050-084X

ISSN

2050-084X

Publication Date

August 2018

Volume

7

Start / End Page

e33051

Related Subject Headings

  • Time-Lapse Imaging
  • Mice, Inbred C57BL
  • Mice
  • Lymph Nodes
  • Germinal Center
  • Clonal Selection, Antigen-Mediated
  • Clonal Evolution
  • Cells, Cultured
  • Cell Movement
  • B-Lymphocytes
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Firl, D. J., Degn, S. E., Padera, T., & Carroll, M. C. (2018). Capturing change in clonal composition amongst single mouse germinal centers. ELife, 7, e33051. https://doi.org/10.7554/elife.33051
Firl, Daniel J., Soren E. Degn, Timothy Padera, and Michael C. Carroll. “Capturing change in clonal composition amongst single mouse germinal centers.ELife 7 (August 2018): e33051. https://doi.org/10.7554/elife.33051.
Firl DJ, Degn SE, Padera T, Carroll MC. Capturing change in clonal composition amongst single mouse germinal centers. eLife. 2018 Aug;7:e33051.
Firl, Daniel J., et al. “Capturing change in clonal composition amongst single mouse germinal centers.ELife, vol. 7, Aug. 2018, p. e33051. Epmc, doi:10.7554/elife.33051.
Firl DJ, Degn SE, Padera T, Carroll MC. Capturing change in clonal composition amongst single mouse germinal centers. eLife. 2018 Aug;7:e33051.

Published In

eLife

DOI

EISSN

2050-084X

ISSN

2050-084X

Publication Date

August 2018

Volume

7

Start / End Page

e33051

Related Subject Headings

  • Time-Lapse Imaging
  • Mice, Inbred C57BL
  • Mice
  • Lymph Nodes
  • Germinal Center
  • Clonal Selection, Antigen-Mediated
  • Clonal Evolution
  • Cells, Cultured
  • Cell Movement
  • B-Lymphocytes