STAT3 Regulates Self-Renewal of Adult Muscle Satellite Cells during Injury-Induced Muscle Regeneration.

Published

Journal Article

Recent studies have shown that STAT3 negatively regulates the proliferation of muscle satellite cells (MuSCs) and injury-induced muscle regeneration. These studies have been largely based on STAT3 inhibitors, which may produce off-target effects and are not cell type-specific in vivo. Here, we examine the role of STAT3 in MuSCs using two different mouse models: a MuSC-specific Stat3 knockout line and a Stat3 (MuSC-specific)/dystrophin (Dmd) double knockout (dKO) line. Stat3(-/-) MuSCs from both mutant lines were defective in proliferation. Moreover, in both mutant strains, the MuSC pool shrank, and regeneration was compromised after injury, with defects more pronounced in dKO mice along with severe muscle inflammation and fibrosis. We analyzed the transcriptomes of MuSCs from dKO and Dmd(-/-) control mice and identified multiple STAT3 target genes, including Pax7. Collectively, our work reveals a critical role of STAT3 in adult MuSCs that regulates their self-renewal during injury-induced muscle regeneration.

Full Text

Duke Authors

Cited Authors

  • Zhu, H; Xiao, F; Wang, G; Wei, X; Jiang, L; Chen, Y; Zhu, L; Wang, H; Diao, Y; Wang, H; Ip, NY; Cheung, TH; Wu, Z

Published Date

  • August 23, 2016

Published In

Volume / Issue

  • 16 / 8

Start / End Page

  • 2102 - 2115

PubMed ID

  • 27524611

Pubmed Central ID

  • 27524611

Electronic International Standard Serial Number (EISSN)

  • 2211-1247

Digital Object Identifier (DOI)

  • 10.1016/j.celrep.2016.07.041

Language

  • eng

Conference Location

  • United States