Skip to main content

miR-203, a tumor suppressor frequently down-regulated by promoter hypermethylation in rhabdomyosarcoma.

Publication ,  Journal Article
Diao, Y; Guo, X; Jiang, L; Wang, G; Zhang, C; Wan, J; Jin, Y; Wu, Z
Published in: J Biol Chem
January 3, 2014

Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma found in children and young adults. It is characterized by the expression of a number of skeletal muscle-specific proteins, including MyoD and muscle α-actin. However, unlike normal myoblasts, RMS cells differentiate poorly both in vivo and in culture. As microRNAs are known to regulate tumorigenesis, intensive efforts have been made to identify microRNAs that are involved in RMS development. In this work, we found that miR-203 was frequently down-regulated by promoter hypermethylation in both RMS cell lines and RMS biopsies and could be reactivated by DNA-demethylating agents. Re-expression of miR-203 in RMS cells inhibited their migration and proliferation and promoted terminal myogenic differentiation. Mechanistically, miR-203 exerts its tumor-suppressive effect by directly targeting p63 and leukemia inhibitory factor receptor in RMS cells, which promotes myogenic differentiation by inhibiting the Notch and the JAK1/STAT1/STAT3 pathways, respectively. Our work reveals that miR-203 functions as a tumor suppressor in RMS development.

Duke Scholars

Altmetric Attention Stats
Dimensions Citation Stats

Published In

J Biol Chem

DOI

EISSN

1083-351X

Publication Date

January 3, 2014

Volume

289

Issue

1

Start / End Page

529 / 539

Location

United States

Related Subject Headings

  • Tumor Suppressor Proteins
  • Transcription Factors
  • Signal Transduction
  • STAT3 Transcription Factor
  • STAT1 Transcription Factor
  • Rhabdomyosarcoma
  • Receptors, OSM-LIF
  • Receptors, Notch
  • Promoter Regions, Genetic
  • Neoplasm Proteins
 

Citation

APA
Chicago
ICMJE
MLA
NLM
Diao, Y., Guo, X., Jiang, L., Wang, G., Zhang, C., Wan, J., … Wu, Z. (2014). miR-203, a tumor suppressor frequently down-regulated by promoter hypermethylation in rhabdomyosarcoma. J Biol Chem, 289(1), 529–539. https://doi.org/10.1074/jbc.M113.494716
Diao, Yarui, Xing Guo, Lei Jiang, Gang Wang, Chao Zhang, Jun Wan, Yan Jin, and Zhenguo Wu. “miR-203, a tumor suppressor frequently down-regulated by promoter hypermethylation in rhabdomyosarcoma.J Biol Chem 289, no. 1 (January 3, 2014): 529–39. https://doi.org/10.1074/jbc.M113.494716.
Diao Y, Guo X, Jiang L, Wang G, Zhang C, Wan J, et al. miR-203, a tumor suppressor frequently down-regulated by promoter hypermethylation in rhabdomyosarcoma. J Biol Chem. 2014 Jan 3;289(1):529–39.
Diao, Yarui, et al. “miR-203, a tumor suppressor frequently down-regulated by promoter hypermethylation in rhabdomyosarcoma.J Biol Chem, vol. 289, no. 1, Jan. 2014, pp. 529–39. Pubmed, doi:10.1074/jbc.M113.494716.
Diao Y, Guo X, Jiang L, Wang G, Zhang C, Wan J, Jin Y, Wu Z. miR-203, a tumor suppressor frequently down-regulated by promoter hypermethylation in rhabdomyosarcoma. J Biol Chem. 2014 Jan 3;289(1):529–539.

Published In

J Biol Chem

DOI

EISSN

1083-351X

Publication Date

January 3, 2014

Volume

289

Issue

1

Start / End Page

529 / 539

Location

United States

Related Subject Headings

  • Tumor Suppressor Proteins
  • Transcription Factors
  • Signal Transduction
  • STAT3 Transcription Factor
  • STAT1 Transcription Factor
  • Rhabdomyosarcoma
  • Receptors, OSM-LIF
  • Receptors, Notch
  • Promoter Regions, Genetic
  • Neoplasm Proteins