Primary Cilia Signaling Shapes the Development of Interneuronal Connectivity.

Published

Journal Article

Appropriate growth and synaptic integration of GABAergic inhibitory interneurons are essential for functional neural circuits in the brain. Here, we demonstrate that disruption of primary cilia function following the selective loss of ciliary GTPase Arl13b in interneurons impairs interneuronal morphology and synaptic connectivity, leading to altered excitatory/inhibitory activity balance. The altered morphology and connectivity of cilia mutant interneurons and the functional deficits are rescued by either chemogenetic activation of ciliary G-protein-coupled receptor (GPCR) signaling or the selective induction of Sstr3, a ciliary GPCR, in Arl13b-deficient cilia. Our results thus define a specific requirement for primary cilia-mediated GPCR signaling in interneuronal connectivity and inhibitory circuit formation.

Full Text

Duke Authors

Cited Authors

  • Guo, J; Otis, JM; Higginbotham, H; Monckton, C; Cheng, J; Asokan, A; Mykytyn, K; Caspary, T; Stuber, GD; Anton, ES

Published Date

  • August 7, 2017

Published In

Volume / Issue

  • 42 / 3

Start / End Page

  • 286 - 300.e4

PubMed ID

  • 28787594

Pubmed Central ID

  • 28787594

Electronic International Standard Serial Number (EISSN)

  • 1878-1551

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2017.07.010

Language

  • eng

Conference Location

  • United States