Optical Control of CRISPR/Cas9 Gene Editing.

Journal Article (Journal Article)

The CRISPR/Cas9 system has emerged as an important tool in biomedical research for a wide range of applications, with significant potential for genome engineering and gene therapy. In order to achieve conditional control of the CRISPR/Cas9 system, a genetically encoded light-activated Cas9 was engineered through the site-specific installation of a caged lysine amino acid. Several potential lysine residues were identified as viable caging sites that can be modified to optically control Cas9 function, as demonstrated through optical activation and deactivation of both exogenous and endogenous gene function.

Full Text

Duke Authors

Cited Authors

  • Hemphill, J; Borchardt, EK; Brown, K; Asokan, A; Deiters, A

Published Date

  • May 6, 2015

Published In

Volume / Issue

  • 137 / 17

Start / End Page

  • 5642 - 5645

PubMed ID

  • 25905628

Pubmed Central ID

  • PMC4919123

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

Digital Object Identifier (DOI)

  • 10.1021/ja512664v


  • eng

Conference Location

  • United States