Production of recombinant adeno-associated viral vectors for in vitro and in vivo use.

Journal Article (Journal Article;Review)

Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection in many different cell types and its ability to persist and lead to long-term gene expression. The vector is also a valuable tool in molecular biology experiments. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free, recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors.

Full Text

Duke Authors

Cited Authors

  • Choi, VW; Asokan, A; Haberman, RA; Samulski, RJ

Published Date

  • April 2007

Published In

Volume / Issue

  • Chapter 16 /

Start / End Page

  • Unit - 16.25

PubMed ID

  • 18265393

Electronic International Standard Serial Number (EISSN)

  • 1934-3647

Digital Object Identifier (DOI)

  • 10.1002/0471142727.mb1625s78


  • eng

Conference Location

  • United States