Production of recombinant adeno-associated viral vectors and use in in vitro and in vivo administration.

Published

Journal Article

Adeno-associated virus is a nonpathogenic human virus that has been developed into a gene-delivery vector due to its high efficiency of infection for many different cell types and its ability to persist and lead to long-term gene expression. This unit describes efficient methods to generate high-titer, research-grade, adenovirus-free recombinant single-stranded and self-complementary adeno-associated virus in various serotypes, along with methods to quantify the viral vectors. Two detailed methods are provided for viral vector delivery into the rodent brain and spinal cord, and for histological detection of transgene expression of GFP.

Full Text

Duke Authors

Cited Authors

  • Gray, SJ; Choi, VW; Asokan, A; Haberman, RA; McCown, TJ; Samulski, RJ

Published Date

  • October 2011

Published In

Volume / Issue

  • Chapter 4 /

Start / End Page

  • Unit - 4.17

PubMed ID

  • 21971848

Pubmed Central ID

  • 21971848

Electronic International Standard Serial Number (EISSN)

  • 1934-8576

Digital Object Identifier (DOI)

  • 10.1002/0471142301.ns0417s57

Language

  • eng

Conference Location

  • United States