Peptide affinity reagents for AAV capsid recognition and purification.
Published
Journal Article
We report the discovery of AAV capsid-binding peptides identified through phage panning. The heptapeptide motif GYVSRHP selectively recognized AAV serotype 8 capsids and blocked transduction in vitro. Recombinant AAV8 vectors were purified directly from crude cell lysate and supernatant through sequential application of peptide affinity and anion exchange chromatography. Peptide affinity reagents may serve as useful alternatives to monoclonal antibodies in AAV capsid recognition, and offer readily scalable solutions for purification of clinical grade AAV vectors.
Full Text
Duke Authors
Cited Authors
- Pulicherla, N; Asokan, A
Published Date
- October 2011
Published In
Volume / Issue
- 18 / 10
Start / End Page
- 1020 - 1024
PubMed ID
- 21490687
Pubmed Central ID
- 21490687
Electronic International Standard Serial Number (EISSN)
- 1476-5462
Digital Object Identifier (DOI)
- 10.1038/gt.2011.46
Language
- eng
Conference Location
- England