Peptide affinity reagents for AAV capsid recognition and purification.

Published

Journal Article

We report the discovery of AAV capsid-binding peptides identified through phage panning. The heptapeptide motif GYVSRHP selectively recognized AAV serotype 8 capsids and blocked transduction in vitro. Recombinant AAV8 vectors were purified directly from crude cell lysate and supernatant through sequential application of peptide affinity and anion exchange chromatography. Peptide affinity reagents may serve as useful alternatives to monoclonal antibodies in AAV capsid recognition, and offer readily scalable solutions for purification of clinical grade AAV vectors.

Full Text

Duke Authors

Cited Authors

  • Pulicherla, N; Asokan, A

Published Date

  • October 2011

Published In

Volume / Issue

  • 18 / 10

Start / End Page

  • 1020 - 1024

PubMed ID

  • 21490687

Pubmed Central ID

  • 21490687

Electronic International Standard Serial Number (EISSN)

  • 1476-5462

International Standard Serial Number (ISSN)

  • 0969-7128

Digital Object Identifier (DOI)

  • 10.1038/gt.2011.46

Language

  • eng