Metabolic glycan labeling and chemoselective functionalization of native biomaterials.

Published

Journal Article

Decellularized native extracellular matrix (ECM) biomaterials are widely used in tissue engineering and have reached clinical application as biomesh implants. To enhance their regenerative properties and postimplantation performance, ECM biomaterials could be functionalized via immobilization of bioactive molecules. To facilitate ECM functionalization, we developed a metabolic glycan labeling approach using physiologic pathways to covalently incorporate click-reactive azide ligands into the native ECM of a wide variety of rodent tissues and organs in vivo, and into the ECM of isolated rodent and porcine lungs cultured ex vivo. The incorporated azides within the ECM were preserved after decellularization and served as chemoselective ligands for subsequent bioconjugation via click chemistry. As proof of principle, we generated alkyne-modified heparin, immobilized it onto azide-incorporated acellular lungs, and demonstrated its bioactivity by Antithrombin III immobilization and Factor Xa inhibition. The herein reported metabolic glycan labeling approach represents a novel platform technology for manufacturing click-reactive native ECM biomaterials, thereby enabling efficient and chemoselective functionalization of these materials to facilitate tissue regeneration and repair.

Full Text

Duke Authors

Cited Authors

  • Ren, X; Evangelista-Leite, D; Wu, T; Rajab, TK; Moser, PT; Kitano, K; Economopoulos, KP; Gorman, DE; Bloom, JP; Tan, JJ; Gilpin, SE; Zhou, H; Mathisen, DJ; Ott, HC

Published Date

  • November 2018

Published In

Volume / Issue

  • 182 /

Start / End Page

  • 127 - 134

PubMed ID

  • 30118980

Pubmed Central ID

  • 30118980

Electronic International Standard Serial Number (EISSN)

  • 1878-5905

International Standard Serial Number (ISSN)

  • 0142-9612

Digital Object Identifier (DOI)

  • 10.1016/j.biomaterials.2018.08.012

Language

  • eng