High-throughput cell focusing and separation via acoustofluidic tweezers.

Journal Article (Journal Article)

Separation of particles and cells is an important function in many biological and biomedical protocols. Although a variety of microfluidic-based techniques have been developed so far, there is clearly still a demand for a precise, fast, and biocompatible method for separation of microparticles and cells. By combining acoustics and hydrodynamics, we have developed a method which we integrated into three-dimensional acoustofluidic tweezers (3D-AFT) to rapidly and efficiently separate microparticles and cells into multiple high-purity fractions. Compared with other acoustophoresis methods, this 3D-AFT method significantly increases the throughput by an order of magnitude, is label-free and gently handles the sorted cells. We demonstrate not only the separation of 10, 12, and 15 micron particles at a throughput up to 500 μl min-1 using this 3D-AFT method, but also the separation of erythrocytes, leukocytes, and cancer cells. This 3D-AFT method is able to meet various separation demands thus offering a viable alternative with potential for clinical applications.

Full Text

Duke Authors

Cited Authors

  • Wu, M; Chen, K; Yang, S; Wang, Z; Huang, P-H; Mai, J; Li, Z-Y; Huang, TJ

Published Date

  • September 2018

Published In

Volume / Issue

  • 18 / 19

Start / End Page

  • 3003 - 3010

PubMed ID

  • 30131991

Pubmed Central ID

  • PMC6203445

Electronic International Standard Serial Number (EISSN)

  • 1473-0189

International Standard Serial Number (ISSN)

  • 1473-0197

Digital Object Identifier (DOI)

  • 10.1039/c8lc00434j


  • eng