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The nuclear receptor steroidogenic factor 1 acts at multiple levels of the reproductive axis.

Publication ,  Journal Article
Ingraham, HA; Lala, DS; Ikeda, Y; Luo, X; Shen, WH; Nachtigal, MW; Abbud, R; Nilson, JH; Parker, KL
Published in: Genes Dev
October 1, 1994

Steroidogenic factor 1 (SF-1), an orphan nuclear receptor, regulates the enzymes that produce sex steroids, and disruption of the Ftz-F1 gene encoding SF-1 precludes adrenal and gonadal development. We now study the role of SF-1 at other levels of the hypothalamic/pituitary/gonadal axis. In Ftz-F1-disrupted mice, immunohistochemical analyses with antibodies against pituitary trophic hormones showed a selective loss of gonadotrope-specific markers, supporting the role of SF-1 in gonadotrope function. In situ hybridization analyses confirmed these results; pituitaries from Ftz-F1-disrupted mice lacked transcripts for three gonadotrope-specific markers (LH beta, FSH beta, and the receptor for gonadotropin-releasing hormone), whereas they exhibited decreased but detectable expression of the alpha-subunit of glycoprotein hormones. SF-1 transcripts in the developing mouse pituitary, which first became detectable at embryonic day 13.5-14.5, preceded the appearance of FSH beta and LH beta transcripts. In adult rat pituitary cells, SF-1 transcripts colocalized with immunoreactivity for the gonadotrope-specific LH. Finally, SF-1 interacted with a previously defined promoter element in the glycoprotein hormone alpha-subunit gene, providing a possible mechanism for the impaired gonadotropin expression in Ftz-F1-disrupted mice. These studies establish novel roles of this orphan nuclear receptor in reproductive function.

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Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

October 1, 1994

Volume

8

Issue

19

Start / End Page

2302 / 2312

Location

United States

Related Subject Headings

  • Transcription Factors
  • Steroidogenic Factor 1
  • Reproduction
  • Receptors, Cytoplasmic and Nuclear
  • Rats
  • RNA, Messenger
  • Pituitary Gland
  • Molecular Sequence Data
  • Mice, Mutant Strains
  • Mice
 

Citation

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Ingraham, H. A., Lala, D. S., Ikeda, Y., Luo, X., Shen, W. H., Nachtigal, M. W., … Parker, K. L. (1994). The nuclear receptor steroidogenic factor 1 acts at multiple levels of the reproductive axis. Genes Dev, 8(19), 2302–2312. https://doi.org/10.1101/gad.8.19.2302
Ingraham, H. A., D. S. Lala, Y. Ikeda, X. Luo, W. H. Shen, M. W. Nachtigal, R. Abbud, J. H. Nilson, and K. L. Parker. “The nuclear receptor steroidogenic factor 1 acts at multiple levels of the reproductive axis.Genes Dev 8, no. 19 (October 1, 1994): 2302–12. https://doi.org/10.1101/gad.8.19.2302.
Ingraham HA, Lala DS, Ikeda Y, Luo X, Shen WH, Nachtigal MW, et al. The nuclear receptor steroidogenic factor 1 acts at multiple levels of the reproductive axis. Genes Dev. 1994 Oct 1;8(19):2302–12.
Ingraham, H. A., et al. “The nuclear receptor steroidogenic factor 1 acts at multiple levels of the reproductive axis.Genes Dev, vol. 8, no. 19, Oct. 1994, pp. 2302–12. Pubmed, doi:10.1101/gad.8.19.2302.
Ingraham HA, Lala DS, Ikeda Y, Luo X, Shen WH, Nachtigal MW, Abbud R, Nilson JH, Parker KL. The nuclear receptor steroidogenic factor 1 acts at multiple levels of the reproductive axis. Genes Dev. 1994 Oct 1;8(19):2302–2312.

Published In

Genes Dev

DOI

ISSN

0890-9369

Publication Date

October 1, 1994

Volume

8

Issue

19

Start / End Page

2302 / 2312

Location

United States

Related Subject Headings

  • Transcription Factors
  • Steroidogenic Factor 1
  • Reproduction
  • Receptors, Cytoplasmic and Nuclear
  • Rats
  • RNA, Messenger
  • Pituitary Gland
  • Molecular Sequence Data
  • Mice, Mutant Strains
  • Mice