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Characterization of the mouse FTZ-F1 gene, which encodes a key regulator of steroid hydroxylase gene expression.

Publication ,  Journal Article
Ikeda, Y; Lala, DS; Luo, X; Kim, E; Moisan, MP; Parker, KL
Published in: Mol Endocrinol
July 1993

The cytochrome P450 steroid hydroxylases are coordinately regulated by steroidogenic factor 1 (SF-1), a protein expressed selectively in steroidogenic cells. Based on its expression in steroidogenic tissues and DNA-binding specificity, we isolated a putative SF-1 cDNA from an adrenocortical cDNA library. As evidence that this cDNA encodes SF-1, we now show that it is selectively expressed in steroidogenic cells, that an antiserum against its protein product specifically abolishes the SF-1-related gel-shift complex, and that its coexpression increases promoter activity of the 21-hydroxylase 5'-flanking region in transfection experiments. Sequence analyses of the SF-1 cDNA revealed that it is the mouse homolog of fushi tarazu factor I (FTZ-F1), a nuclear receptor that regulates the fushi tarazu homeobox gene in Drosophila. A second FTZ-F1 homolog, embryonal long terminal repeat-binding protein (ELP), was recently isolated from embryonal carcinoma cells. SF-1 and ELP cDNAs are virtually identical for 1017 base pairs, including putative DNA-binding domains, but diverge at their 5'- and 3'-ends. One genomic clone contained both SF-1- and ELP-specific sequences, confirming their origin from a single gene. Characterization of this gene defined shared exons encoding common regions and alternative promoters and 3'-exons leading to differences between the two FTZ-F1 transcripts. We used in situ hybridization with transcript-specific probes to study the ontogeny of SF-1 and ELP expression. ELP transcripts were not detected from embryonic day 8 to adult, consistent with its previous isolation from embryonal carcinoma cells and its postulated role in early embryonic development.(ABSTRACT TRUNCATED AT 250 WORDS)

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Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

July 1993

Volume

7

Issue

7

Start / End Page

852 / 860

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Steroidogenic Factor 1
  • Steroid Hydroxylases
  • Steroid 21-Hydroxylase
  • Repressor Proteins
  • Receptors, Cytoplasmic and Nuclear
  • Promoter Regions, Genetic
  • Neoplasms, Experimental
 

Citation

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Ikeda, Y., Lala, D. S., Luo, X., Kim, E., Moisan, M. P., & Parker, K. L. (1993). Characterization of the mouse FTZ-F1 gene, which encodes a key regulator of steroid hydroxylase gene expression. Mol Endocrinol, 7(7), 852–860. https://doi.org/10.1210/mend.7.7.8413309
Ikeda, Y., D. S. Lala, X. Luo, E. Kim, M. P. Moisan, and K. L. Parker. “Characterization of the mouse FTZ-F1 gene, which encodes a key regulator of steroid hydroxylase gene expression.Mol Endocrinol 7, no. 7 (July 1993): 852–60. https://doi.org/10.1210/mend.7.7.8413309.
Ikeda Y, Lala DS, Luo X, Kim E, Moisan MP, Parker KL. Characterization of the mouse FTZ-F1 gene, which encodes a key regulator of steroid hydroxylase gene expression. Mol Endocrinol. 1993 Jul;7(7):852–60.
Ikeda, Y., et al. “Characterization of the mouse FTZ-F1 gene, which encodes a key regulator of steroid hydroxylase gene expression.Mol Endocrinol, vol. 7, no. 7, July 1993, pp. 852–60. Pubmed, doi:10.1210/mend.7.7.8413309.
Ikeda Y, Lala DS, Luo X, Kim E, Moisan MP, Parker KL. Characterization of the mouse FTZ-F1 gene, which encodes a key regulator of steroid hydroxylase gene expression. Mol Endocrinol. 1993 Jul;7(7):852–860.

Published In

Mol Endocrinol

DOI

ISSN

0888-8809

Publication Date

July 1993

Volume

7

Issue

7

Start / End Page

852 / 860

Location

United States

Related Subject Headings

  • Tumor Cells, Cultured
  • Transcription, Genetic
  • Transcription Factors
  • Steroidogenic Factor 1
  • Steroid Hydroxylases
  • Steroid 21-Hydroxylase
  • Repressor Proteins
  • Receptors, Cytoplasmic and Nuclear
  • Promoter Regions, Genetic
  • Neoplasms, Experimental